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PMID: 16116040 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Specific roles of 5' RNA secondary structures in stabilizing transcripts in chloroplasts.

Nucleic acids research ·Vol. 33 ·No. 15 ·2005-00-00 ·Pages 4754-61

Suay L, Salvador ML, Abesha E, Klein U

Abstract

RNA secondary structures, e.g. stem-loops that are often found at the 5' and 3' ends of mRNAs, are in many cases known to be crucial for transcript stability but their role in prolonging the lifetime of transcripts remains elusive. In this study we show for an essential RNA-stabilizing stem-loop at the 5' end of rbcL gene transcripts in Chlamydomonas that it neither prevents ribonucleases from binding to the RNA nor impedes their movement along the RNA strand. The stem-loop has a formative function in that it mediates folding of a short sequence around its base into a specific RNA conformation, consisting of a helical and single-stranded region, i.e. the real structure required for longevity of rbcL transcripts in chloroplasts. Disturbing this structure renders transcripts completely unstable, even if the sequence of this element is not altered. The requirement of a specific 5' sequence and structure for RNA longevity suggests an interaction of this element with a trans-acting factor that protects transcripts from rapid degradation in chloroplasts.

MeSH Terms
5' Untranslated Regions/chemistry Animals Base Sequence Chlamydomonas/genetics Molecular Sequence Data Nucleic Acid Conformation RNA Stability RNA, Chloroplast/chemistry,metabolism RNA, Messenger/chemistry,metabolism Ribulose-Bisphosphate Carboxylase/genetics,metabolism
Chemicals
5' Untranslated Regions RNA, Chloroplast RNA, Messenger RbcL protein, plastid Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Suay Loreto
Department of Biochemistry and Molecular Biology, University of Valencia Dr Moliner 50, Burjassot, Valencia 46100, Spain.
Salvador Maria L
Abesha Emnet
Klein Uwe
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gki760
Published
2005-00-00
Epub
2005-00-22
Pages
4754-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1188514
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gki760 PMC Full Text View journal details
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