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PMID: 12884008 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.

Molecular genetics and genomics : MGG ·Vol. 269 ·No. 5 ·2003-08-00 ·Pages 706-14

Hambraeus G, von Wachenfeldt C, Hederstedt L

Abstract

We have used DNA microarrays to survey rates of mRNA decay on a genomic scale in early stationary-phase cultures of Bacillus subtilis. The decay rates for mRNAs corresponding to about 1500 genes could be estimated. About 80% of these mRNAs had a half-life of less than 7 min. More than 30 mRNAs, including both mono- and polycistronic transcripts, were found to be extremely stable, i.e. to have a half-life of > or =15 min. Only two such transcripts were known previously in B. subtilis. The results provide the first overview of mRNA decay rates in a gram-positive bacterium and help to identify polycistronic operons. We could find no obvious correlation between the stability of an mRNA and the function of the encoded protein. We have also not found any general features in the 5' regions of mRNAs that distinguish stable from unstable transcripts. The identified set of extremely stable mRNAs may be useful in the construction of stable recombinant genes for the overproduction of biomolecules in Bacillus species.

MeSH Terms
Bacillus subtilis/genetics Genome Genome, Bacterial Half-Life Oligonucleotide Array Sequence Analysis RNA Stability RNA, Bacterial RNA, Messenger
Chemicals
RNA, Bacterial RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hambraeus G
Department of Cell and Organism Biology, Lund University, Sölvegatan 35, 223 62 Lund, Sweden.
von Wachenfeldt C
Hederstedt L
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-003-0883-6
Published
2003-08-00
Epub
2003-00-23
Pages
706-14
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
External Links
PubMed source DOI: 10.1007/s00438-003-0883-6 View journal details
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