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PMID: 160566 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Gene for the RNA polymerase sigma subunit mapped in Salmonella typhimurium and Escherichia coli by cloning and deletion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 12 ·1979-12-00 ·Pages 6510-4

Scaife JG, Heilig JS, Rowen L, Calendar R

Abstract

The genes for the RNA polymerase sigma subunit (rpoD) and DNA primase (dnaG) of Salmonella typhimurium have been cloned into lambda vectors. Combined restriction, deletion and functional analysis of the cloned fragment allows us to map the genes precisely on the fragment, establishes the direction in which rpoD is transcribed, and reveals the existence of at least one new gene in the vicinity. A closely homologous, smaller fragment of Escherichia coli DNA, also cloned into lambda, contains rpoD and at least part of dnaG.

MeSH Terms
Bacteriophage lambda/genetics Chromosome Deletion Chromosome Mapping Chromosomes, Bacterial/ultrastructure Cloning, Molecular DNA, Recombinant DNA-Directed RNA Polymerases/genetics Escherichia coli/genetics Genes Genetic Linkage Macromolecular Substances Operon Salmonella typhimurium/genetics
Chemicals
DNA, Recombinant Macromolecular Substances DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Scaife J G
Heilig J S
Rowen L
Calendar R
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.12.6510
Published
1979-12-00
Pages
6510-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411895
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.12.6510 PMC Full Text View journal details
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