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PMID: 362159 Published · ppublish English Journal Article

RNA polymerase mutant with altered sigma factor in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 165 ·No. 1 ·1978-09-20 ·Pages 1-6

Nakamura Y

Abstract

A structural gene for sigma factor (rpoD) of DNA-dependent RNA polymerase (RNA nucleotidyltransferase; nucleoside-triphosphate: RNA nucleotidyltransferase, E.C. 2.7.7.6) was mapped precisely by a set of F' factors including those already published (Proc. Natl. Acad. Sci. USA. 74, 1831-1835 (1977)). Based on the result that rpoD is located at the dnaG-uxaAC region, a number of mutants containing a temperature-sensitive mutation at or near the uxaA gene were isolated by localized mutagenesis. One of these mutants was found to produce RNA polymerase altered in both thermostability and optimum salt concentration as a result of structural alteration of sigma factor. This mutation, U303, maps at 66 min on the genetic map of E. coli, near the dnaG locus, and affects normal growth of cells.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial DNA-Directed RNA Polymerases/genetics,isolation & purification Escherichia coli/genetics Genes Mutation Phenotype Sigma Factor/genetics,isolation & purification Transcription Factors/genetics
Chemicals
Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Nakamura Y
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25 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270369
Published
1978-09-20
Pages
1-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270369
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