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PMID: 1588974 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A transcriptionally active DNA-binding site for human p53 protein complexes.

Molecular and cellular biology ·Vol. 12 ·No. 6 ·1992-06-00 ·Pages 2866-71

Funk WD, Pak DT, Karas RH, Wright WE, Shay JW

Abstract

Recent studies have demonstrated transcriptional activation domains within the tumor suppressor protein p53, while others have described specific DNA-binding sites for p53, implying that the protein may act as a transcriptional regulatory factor. We have used a reiterative selection procedure (CASTing: cyclic amplification and selection of targets) to identify new specific binding sites for p53, using nuclear extracts from normal human fibroblasts as the source of p53 protein. The preferred consensus is the palindrome GGACATGCCCGGGCATGTCC. In vitro-translated p53 binds to this sequence only when mixed with nuclear extracts, suggesting that p53 may bind DNA after posttranslational modification or as a complex with other protein partners. When placed upstream of a reporter construct, this sequence promotes p53-dependent transcription in transient transfection assays.

MeSH Terms
Base Sequence Binding Sites Consensus Sequence DNA-Binding Proteins/metabolism Gene Expression Regulation Humans In Vitro Techniques Macromolecular Substances Molecular Sequence Data Nuclear Proteins/metabolism Regulatory Sequences, Nucleic Acid Transcription, Genetic Tumor Suppressor Protein p53/metabolism
Chemicals
DNA-Binding Proteins Macromolecular Substances Nuclear Proteins Tumor Suppressor Protein p53
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Funk W D
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235-9039.
Pak D T
Karas R H
Wright W E
Shay J W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.6.2866-2871.1992
Published
1992-06-00
Pages
2866-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364481
Subset
IM
Grants
NIA NIH HHS · AG07992 · United States
NCI NIH HHS · CA50195 · United States
Databases
GENBANK
X63571
External Links
PubMed source DOI: 10.1128/mcb.12.6.2866-2871.1992 PMC Full Text View journal details
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