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PMID: 15883881 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Comparison of the PhoPQ regulon in Escherichia coli and Salmonella typhimurium.

Journal of molecular evolution ·Vol. 60 ·No. 4 ·2005-04-00 ·Pages 462-74

Monsieurs P, De Keersmaecker S, Navarre WW, Bader MW, De Smet F, McClelland M, Fang FC, De Moor B, Vanderleyden J, Marchal K

Abstract

The PhoPQ two-component system acts as a transcriptional regulator that responds to Mg(2+) starvation both in Escherichia coli and Salmonella typhimurium (Garcia et al. 1996; Kato et al. 1999). By monitoring the availability of extracellular Mg(2+), this two-component system allows S. typhimurium to sense the transition from an extracellular environment to a subcellular location. Concomitantly with this transition, a set of virulence factors essential for survival in the intracellular environment is activated by the PhoPQ system (Groisman et al. 1989; Miller et al. 1989). Compared to nonpathogenic strains, such as E. coli K12, the PhoPQ regulon in pathogens must contain target genes specifically contributing to the virulence phenotype. To verify this hypothesis, we compared the composition of the PhoPQ regulon between E. coli and S. typhimurium using a combination of expression experiments and motif data. PhoPQ-dependent genes in both organisms were identified from PhoPQ-related microarray experiments. To distinguish between direct and indirect targets, we searched for the presence of the regulatory motif in the promoter region of the identified PhoPQ-dependent genes. This allowed us to reconstruct the direct PhoPQ-dependent regulons in E. coli K12 and S. typhimurium LT2. Comparison of both regulons revealed a very limited overlap of PhoPQ-dependent genes between both organisms. These results suggest that the PhoPQ system has acquired a specialized function during evolution in each of these closely related species that allows adaptation to the specificities of their lifestyles (e.g., pathogenesis in S. typhimurium).

MeSH Terms
Bacterial Proteins/genetics Base Sequence Escherichia coli/genetics Escherichia coli Proteins/genetics Oligonucleotide Array Sequence Analysis Regulon Salmonella typhimurium/genetics Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins Escherichia coli Proteins PhoP protein, E coli PhoQ protein, Bacteria PhoP protein, Bacteria
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Monsieurs Pieter
ESAT-SCD, K.U. Leuven, Kasteelpark Arenberg 10, 3001, Leuven-Heverlee, Belgium.
De Keersmaecker Sigrid
Navarre William W
Bader Martin W
De Smet Frank
McClelland Michael
Fang Ferric C
De Moor Bart
Vanderleyden Jos
Marchal Kathleen
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/s00239-004-0212-7
Published
2005-04-00
Pages
462-74
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
External Links
PubMed source DOI: 10.1007/s00239-004-0212-7 View journal details
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