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PMID: 9311997 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Salmonella selC locus contains a pathogenicity island mediating intramacrophage survival.

The EMBO journal ·Vol. 16 ·No. 17 ·1997-09-01 ·Pages 5376-85

Blanc-Potard AB, Groisman EA

Abstract

Pathogenicity islands are chromosomal clusters of horizontally acquired virulence genes that are often found at tRNA loci. The selC tRNA locus of Escherichia coli has served as the site of integration of two distinct pathogenicity islands which are responsible for converting benign strains into uro- and enteropathogens. Because virulence genes are targeted to the selC locus of E.coli, we investigated the homologous region of the Salmonella typhimurium chromosome for the presence of horizontally acquired sequences. At this site, we identified a 17 kb DNA segment that is both unique to Salmonella and necessary for virulence. This segment harbors a gene, mgtC, that is required for intramacrophage survival and growth in low Mg2+ media. The mgtC locus is regulated by the PhoP/PhoQ two-component system, a major regulator of virulence functions present in both pathogenic and non-pathogenic bacterial species. Cumulatively, our experiments indicate that the ability to replicate in low Mg2+ environments is necessary for Salmonella virulence, and suggest that a similar mechanism is responsible for the dissemination and acquisition of pathogenicity islands in enteric bacteria.

MeSH Terms
Adenosine Triphosphatases/genetics Animals Bacterial Proteins/genetics Base Sequence Carrier Proteins/genetics Cation Transport Proteins Culture Media Escherichia coli/genetics,growth & development Escherichia coli Proteins Female Genes, Bacterial Macrophages/microbiology Magnesium/pharmacology Membrane Transport Proteins Mice Mice, Inbred BALB C Molecular Sequence Data Multigene Family Mutation Operon Phenotype RNA, Transfer/genetics Recombination, Genetic Salmonella Infections, Animal/genetics Salmonella typhimurium/genetics,pathogenicity
Chemicals
Bacterial Proteins Carrier Proteins Cation Transport Proteins Culture Media Escherichia coli Proteins Membrane Transport Proteins RNA, Transfer Adenosine Triphosphatases MgtA protein, E coli MgtA protein, bacteria MgtB protein, Salmonella typhimurium MgtC protein, Salmonella typhimurium Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blanc-Potard A B
Department of Molecular Microbiology, Washington University School of Medicine, 660 S.Euclid Ave, Campus Box 8230, St Louis, MO 63110, USA.
Groisman E A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.17.5376
Published
1997-09-01
Pages
5376-85
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170169
Subset
IM
Grants
NIGMS NIH HHS · GM54900 · United States
Databases
GENBANK
AJ000509, Y13864
External Links
PubMed source DOI: 10.1093/emboj/16.17.5376 PMC Full Text View journal details
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