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PMID: 15805486 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The sulfate transporter SST1 is crucial for symbiotic nitrogen fixation in Lotus japonicus root nodules.

The Plant cell ·Vol. 17 ·No. 5 ·2005-05-00 ·Pages 1625-36

Krusell L, Krause K, Ott T, Desbrosses G, Krämer U, Sato S, Nakamura Y, Tabata S, James EK, Sandal N, Stougaard J, Kawaguchi M, Miyamoto A, Suganuma N, Udvardi MK

Abstract

Symbiotic nitrogen fixation (SNF) by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. Little is known at the molecular level about plant transporters that mediate such exchanges. Several mutants of the model legume Lotus japonicus have been identified that develop nodules with metabolic defects that cannot fix nitrogen efficiently and exhibit retarded growth under symbiotic conditions. Map-based cloning of defective genes in two such mutants, sst1-1 and sst1-2 (for symbiotic sulfate transporter), revealed two alleles of the same gene. The gene is expressed in a nodule-specific manner and encodes a protein homologous with eukaryotic sulfate transporters. Full-length cDNA of the gene complemented a yeast mutant defective in sulfate transport. Hence, the gene was named Sst1. The sst1-1 and sst1-2 mutants exhibited normal growth and development under nonsymbiotic growth conditions, a result consistent with the nodule-specific expression of Sst1. Data from a previous proteomic study indicate that SST1 is located on the symbiosome membrane in Lotus nodules. Together, these results suggest that SST1 transports sulfate from the plant cell cytoplasm to the intracellular rhizobia, where the nutrient is essential for protein and cofactor synthesis, including nitrogenase biosynthesis. This work shows the importance of plant sulfate transport in SNF and the specialization of a eukaryotic transporter gene for this purpose.

MeSH Terms
Amino Acid Sequence Anion Transport Proteins/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Base Sequence Cell Membrane/metabolism Cytoplasm/metabolism DNA, Complementary/analysis,genetics Gene Expression Regulation, Plant/genetics Lotus/metabolism Membrane Transport Proteins/genetics,metabolism Molecular Sequence Data Mutation/genetics Nitrogen/metabolism Nitrogen Fixation/physiology Phylogeny Plant Proteins/genetics,metabolism Plant Roots/metabolism Protein Transport/physiology Sulfate Transporters Sulfates/metabolism Symbiosis/physiology
Chemicals
AST68 protein, Arabidopsis Anion Transport Proteins Arabidopsis Proteins DNA, Complementary Membrane Transport Proteins Plant Proteins Sulfate Transporters Sulfates Nitrogen
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Krusell Lene
Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany.
Krause Katja
Ott Thomas
Desbrosses Guilhem
Krämer Ute
Sato Shusei
Nakamura Yasukazu
Tabata Satoshi
James Euan K
Sandal Niels
Stougaard Jens
Kawaguchi Masayoshi
Miyamoto Ai
Suganuma Norio
Udvardi Michael K
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.030106
Published
2005-05-00
Epub
2005-00-01
Pages
1625-36
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1091779
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.104.030106 PMC Full Text View journal details
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