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PMID: 9055073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of two high affinity sulfate transporters in Saccharomyces cerevisiae.

Genetics ·Vol. 145 ·No. 3 ·1997-03-00 ·Pages 627-35

Cherest H, Davidian JC, Thomas D, Benes V, Ansorge W, Surdin-Kerjan Y

Abstract

Strains resistant to the toxic analogues of sulfate, selenate and chromate have been isolated. Their genetic analysis allowed us to identify four genes. One, called MET28, encodes a transcriptional factor. The three other genes, called SUL1, SUL2 and SUL3, encode proteins involved in sulfate transport. The sequence of Sul1p and Sul2p indicate that they are integral membrane proteins exhibiting, respectively, 11 and 10 transmembrane domains. Moreover, Sul1p and Sul2p share a high degree of similarity. Sulfate transport kinetic studies made with parental and mutant strains show that, as expected from genetic results, Saccharomyces cerevisiae has two high affinity sulfate transport systems. Sul3p has been shown to be involved in the transcriptional regulation of the SUL2 gene.

MeSH Terms
Amino Acid Sequence Anion Transport Proteins Carrier Proteins/genetics Cloning, Molecular Fungal Proteins/genetics Gene Expression Regulation, Fungal Membrane Transport Proteins Methionine/metabolism Molecular Sequence Data Mutagenesis Phenotype Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Sulfates/metabolism Transcription Factors
Chemicals
Anion Transport Proteins Carrier Proteins FZF1 protein, S cerevisiae Fungal Proteins Membrane Transport Proteins SUL2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Sulfates Transcription Factors Methionine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cherest H
Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Davidian J C
Thomas D
Benes V
Ansorge W
Surdin-Kerjan Y
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/145.3.627
Published
1997-03-00
Pages
627-35
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207848
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/145.3.627 PMC Full Text View journal details
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