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PMID: 12196410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A genetic linkage map of the model legume Lotus japonicus and strategies for fast mapping of new loci.

Genetics ·Vol. 161 ·No. 4 ·2002-08-00 ·Pages 1673-83

Sandal N, Krusell L, Radutoiu S, Olbryt M, Pedrosa A, Stracke S, Sato S, Kato T, Tabata S, Parniske M, Bachmair A, Ketelsen T, Stougaard J

Abstract

A genetic map for the model legume Lotus japonicus has been developed. The F(2) mapping population was established from an interspecific cross between L. japonicus and L. filicaulis. A high level of DNA polymorphism between these parents was the source of markers for linkage analysis and the map is based on a framework of amplified fragment length polymorphism (AFLP) markers. Additional markers were generated by restriction fragment length polymorphism (RFLP) and sequence-specific PCR. A total of 524 AFLP markers, 3 RAPD markers, 39 gene-specific markers, 33 microsatellite markers, and six recessive symbiotic mutant loci were mapped. This genetic map consists of six linkage groups corresponding to the six chromosomes in L. japonicus. Fluorescent in situ hybridization (FISH) with selected markers aligned the linkage groups to chromosomes as described in the accompanying article by Pedrosa et al. 2002(this issue). The length of the linkage map is 367 cM and the average marker distance is 0.6 cM. Distorted segregation of markers was found in certain sections of the map and linkage group I could be assembled only by combining colormapping and cytogenetics (FISH). A fast method to position genetic loci employing three AFLP primer combinations yielding 89 markers was developed and evaluated by mapping three symbiotic loci, Ljsym1, Ljsym5, and Ljhar1-3.

MeSH Terms
Chromosome Mapping/methods Genetic Markers Hybridization, Genetic Lotus/genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Analysis, DNA Symbiosis/genetics
Chemicals
Genetic Markers
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Sandal Niels
Laboratory of Gene Expression, Department of Molecular and Structural Biology, University of Aarhus, DK-8000 Aarhus C, Denmark. sandal@biobase.dk
Krusell Lene
Radutoiu Simona
Olbryt Magdalena
Pedrosa Andrea
Stracke Silke
Sato Shusei
Kato Tomohiko
Tabata Satoshi
Parniske Martin
Bachmair Andreas
Ketelsen Tina
Stougaard Jens
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/161.4.1673
Email
sandal@biobase.dk
Published
2002-08-00
Pages
1673-83
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462218
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/161.4.1673 PMC Full Text View journal details
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