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PMID: 15790397 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, P.H.S.

NXT2 is required for embryonic heart development in zebrafish.

BMC developmental biology ·Vol. 5 ·2005-03-24 ·Pages 7

Huang H, Zhang B, Hartenstein PA, Chen JN, Lin S

Abstract

NXT2 is a member of NXT family proteins that are generally involved in exporting nuclear RNA in eukaryotic cells. It is not known if NXT2 has any function in specific biological processes. A zebrafish mutant exhibiting specific heart defects during embryogenesis was generated by animal cloning-mediated retroviral insertions. Molecular analysis indicated that the mutant phenotype was caused by a disruption of NXT2. Whole-mount RNA in situ hybridization showed that NXT2 transcripts were clearly detectable in embryonic heart as well as other tissues. Further analysis revealed that expression level of one form of alternative splicing NXT2 mRNA transcripts was significantly reduced, resulting in deficient myocardial cell differentiation and the malformation of cardiac valve at the atrioventricular boundary. The defects could be reproduced by morpholino anti-sense oligo knockdown of NXT2. NXT2 has a critical role in maintaining morphogenetic integrity of embryonic heart in vertebrate species.

MeSH Terms
Active Transport, Cell Nucleus Alternative Splicing Animals Cell Differentiation/genetics Cloning, Organism DNA Transposable Elements Edema, Cardiac/genetics,pathology Heart/embryology,physiology Heart Defects, Congenital/genetics,pathology Heart Valves/pathology Myocardium/chemistry,cytology,pathology Nuclear Export Signals/genetics,physiology Phenotype RNA, Antisense RNA, Messenger/analysis,genetics Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic Zebrafish/embryology,genetics Zebrafish Proteins/genetics,physiology
Chemicals
DNA Transposable Elements NXT2 protein, zebrafish Nuclear Export Signals RNA, Antisense RNA, Messenger Zebrafish Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huang Haigen
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, California 90095, USA. haigenh@ucla.edu
Zhang Bo
Hartenstein Parvana A
Chen Jau-nian
Lin Shuo
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Article Info
Journal
BMC developmental biology
Abbr.
BMC Dev Biol
ISSN
1471-213X
DOI
10.1186/1471-213X-5-7
Email
haigenh@ucla.edu
Published
2005-03-24
Epub
2005-00-24
Pages
7
Language
English
Region
England
NLM ID
100966973
PMCID
PMC1079804
Subset
IM
Grants
NIDDK NIH HHS · R01 DK065638 · United States
NICHD NIH HHS · R01 HD041367 · United States
NIDDK NIH HHS · R01 DK65638 · United States
NICHD NIH HHS · R01HD41367 · United States
External Links
PubMed source DOI: 10.1186/1471-213X-5-7 PMC Full Text View journal details
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