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PMID: 15709028 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human cytomegalovirus tegument protein ppUL35 is important for viral replication and particle formation.

Journal of virology ·Vol. 79 ·No. 5 ·2005-03-00 ·Pages 3084-96

Schierling K, Buser C, Mertens T, Winkler M

Abstract

The tegument proteins ppUL35 and ppUL82 (pp71) of human cytomegalovirus (HCMV) physically interact and cooperatively activate the major immediate-early transcription. While an HCMV mutant lacking UL82 displayed a multiplicity of infection (MOI)-dependent growth, the biological significance of ppUL35 has not been addressed so far. We generated a mutant virus with a deletion of the UL35 gene. Using an MOI of 0.1, the progeny virus yield of this mutant was reduced by a factor of 1,000; however, when infected at a low MOI (0.01), the gene was essential. Characterization of the replication cycle showed that the mutant virus had two defects: when virus inoculum was standardized by the amount of viral DNA, a reduced immediate-early gene expression was observed, leading to a strongly delayed expression of lytic genes. A second defect was apparent in the virus assembly, as fewer enveloped particles and no dense bodies were present in cells infected with the mutant virus. However, the particles produced by wild-type and mutant viruses did not show significant ultrastructural differences. These results suggest an important role for ppUL35 in immediate-early gene expression and virus assembly.

MeSH Terms
Base Sequence Cell Line Cytomegalovirus/genetics,physiology,ultrastructure DNA, Viral/genetics Gene Deletion Gene Expression Regulation, Viral Genes, Immediate-Early Genes, Viral Humans Microscopy, Electron Mutagenesis Viral Proteins/genetics,physiology Virus Assembly/genetics,physiology Virus Replication/genetics,physiology
Chemicals
DNA, Viral Viral Proteins cytomegalovirus phosphoprotein 71kDa
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schierling Karina
Abteilung Virologie, Universitätsklinikum Ulm, Albert Einstein Allee 11, D-89081 Ulm, Germany. michael.winkler@medizin.uni-ulm.de
Buser Christopher
Mertens Thomas
Winkler Michael
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.79.5.3084-3096.2005
Email
michael.winkler@medizin.uni-ulm.de
Published
2005-03-00
Pages
3084-96
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC548451
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.79.5.3084-3096.2005 PMC Full Text View journal details
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