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PMID: 12970444 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human cytomegalovirus UL99-encoded pp28 is required for the cytoplasmic envelopment of tegument-associated capsids.

Journal of virology ·Vol. 77 ·No. 19 ·2003-10-00 ·Pages 10594-605

Silva MC, Yu QC, Enquist L, Shenk T

Abstract

The human cytomegalovirus UL99-encoded pp28 is a myristylated phosphoprotein that is a constituent of the virion. The pp28 protein is positioned within the tegument of the virus particle, a protein structure that resides between the capsid and envelope. In the infected cell, pp28 is found in a cytoplasmic compartment derived from the Golgi apparatus, where the virus buds into vesicles to acquire its final membrane. We have constructed two mutants of human cytomegalovirus that fail to produce the pp28 protein, a substitution mutant (BADsubUL99) and a point mutant (BADpmUL99), and we have propagated them by complementation in pp28-expressing fibroblasts. Both mutant viruses are profoundly defective for growth in normal fibroblasts; no infectious virus could be detected after infection. Whereas normal levels of viral DNA and late proteins were observed in mutant virus-infected cells, large numbers of tegument-associated capsids accumulated in the cytoplasm that failed to acquire an envelope. We conclude that pp28 is required for the final envelopment of the human cytomegalovirus virion in the cytoplasm.

MeSH Terms
Capsid/physiology Cells, Cultured Cytomegalovirus/physiology Cytoplasm/virology Humans Phosphoproteins/genetics,metabolism,physiology Viral Envelope Proteins/metabolism Viral Matrix Proteins/metabolism Viral Proteins/genetics,physiology Virus Assembly Virus Replication
Chemicals
Phosphoproteins Viral Envelope Proteins Viral Matrix Proteins Viral Proteins cytomegalovirus matrix protein 65kDa glycoprotein B, Simplexvirus human cytomegalovirus protein pp28
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Silva Maria C
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA.
Yu Qian-Chun
Enquist Lynn
Shenk Thomas
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.19.10594-10605.2003
Published
2003-10-00
Pages
10594-605
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC228509
Subset
IM
Grants
NCI NIH HHS · R01 CA082396 · United States
NCI NIH HHS · CA82396 · United States
External Links
PubMed source DOI: 10.1128/jvi.77.19.10594-10605.2003 PMC Full Text View journal details
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