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PMID: 11435566 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cytomegalovirus basic phosphoprotein (pUL32) binds to capsids in vitro through its amino one-third.

Journal of virology ·Vol. 75 ·No. 15 ·2001-08-00 ·Pages 6865-73

Baxter MK, Gibson W

Abstract

The cytomegalovirus (CMV) basic phosphoprotein (BPP) is a component of the tegument. It remains with the nucleocapsid fraction under conditions that remove most other tegument proteins from the virion, suggesting a direct and perhaps tight interaction with the capsid. As a step toward localizing this protein within the molecular structure of the virion and understanding its function during infection, we have investigated the BPP-capsid interaction. In this report we present evidence that the BPP interacts selectively, through its amino one-third, with CMV capsids. Radiolabeled simian CMV (SCMV) BPP, synthesized in vitro, bound to SCMV B-capsids, and C-capsids to a lesser extent, following incubation with either isolated capsids or lysates of infected cells. Human CMV (HCMV) BPP (pUL32) also bound to SCMV capsids, and SCMV BPP likewise bound to HCMV capsids, indicating that the sequence(s) involved is conserved between the two proteins. Analysis of SCMV BPP truncation mutants localized the capsid-binding region to the amino one-third of the molecule--the portion of BPP showing the greatest sequence conservation between the SCMV and HCMV homologs. This general approach may have utility in studying the interactions of other proteins with conformation-dependent binding sites.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Capsid/metabolism Cell Line Chlorocebus aethiops Cytomegalovirus/genetics,metabolism DNA, Viral Haplorhini Humans Molecular Sequence Data Phosphoproteins Sequence Homology, Amino Acid Vero Cells Viral Matrix Proteins/genetics,metabolism
Chemicals
DNA, Viral Phosphoproteins Viral Matrix Proteins pp150 protein, Cytomegalovirus
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baxter M K
Virology Laboratories, Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Gibson W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
2001-08-00
Pages
6865-73
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC114414
Subset
IM
Grants
NIAID NIH HHS · R01 AI013718 · United States
NIAID NIH HHS · R56 AI013718 · United States
NIAID NIH HHS · AI13718 · United States
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GENBANK
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