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PMID: 1569939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding of the estrogen receptor DNA-binding domain to the estrogen response element induces DNA bending.

Molecular and cellular biology ·Vol. 12 ·No. 5 ·1992-05-00 ·Pages 2037-42

Nardulli AM, Shapiro DJ

Abstract

We have used circular permutation analysis to determine whether binding of purified Xenopus laevis estrogen receptor DNA-binding domain (DBD) to a DNA fragment containing an estrogen response element (ERE) causes the DNA to bend. Gel mobility shift assays showed that DBD-DNA complexes formed with fragments containing more centrally located EREs migrated more slowly than complexes formed with fragments containing EREs near the ends of the DNA. DNA bending standards were used to determine that the degree of bending induced by binding of the DBD to an ERE was approximately 34 degrees. A 1.55-fold increase in the degree of bending was observed when two EREs were present in the DNA fragment. These in vitro studies suggest that interaction of nuclear receptors with their hormone response elements in vivo may result in an altered DNA conformation.

MeSH Terms
Animals Base Sequence Binding Sites DNA/isolation & purification,metabolism DNA-Binding Proteins/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Genetic Vectors Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides/metabolism Protein Binding Receptors, Estrogen/isolation & purification,metabolism Regulatory Sequences, Nucleic Acid Restriction Mapping TATA Box Xenopus laevis
Chemicals
DNA-Binding Proteins Oligodeoxyribonucleotides Receptors, Estrogen DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nardulli A M
Department of Biochemistry, University of Illinois, Urbana 61801.
Shapiro D J
References (43)
43 references, click to expand
  1. Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter.
    Cell. 1990 Mar 9;60(5):719-31 PMID: 2155706
  2. The locus of sequence-directed and protein-induced DNA bending.
    Nature. 1984 Apr 5-11;308(5959):509-13 PMID: 6323997
  3. Distinct classes of transcriptional activating domains function by different mechanisms.
    Cell. 1990 Sep 21;62(6):1177-87 PMID: 2205398
  4. An NF1-related vitellogenin activator element mediates transcription from the estrogen-regulated Xenopus laevis vitellogenin promoter.
    J Biol Chem. 1990 May 15;265(14):8176-82 PMID: 2335521
  5. Synergistic activation of transcription by the human estrogen receptor bound to tandem responsive elements.
    EMBO J. 1990 Jul;9(7):2221-31 PMID: 2357968
  6. Synergistic action of the glucocorticoid receptor with transcription factors.
    EMBO J. 1988 Nov;7(11):3389-95 PMID: 2463158
  7. DNA bending is induced by a transcription factor that interacts with the human c-FOS and alpha-actin promoters.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2162-6 PMID: 2494661
  8. Gene regulation by steroid hormones.
    Cell. 1989 Feb 10;56(3):335-44 PMID: 2644044
  9. RNA polymerase and gal repressor bind simultaneously and with DNA bending to the control region of the Escherichia coli galactose operon.
    EMBO J. 1989 Apr;8(4):1247-55 PMID: 2663472
  10. DNA bending is induced in an enhancer by the DNA-binding domain of the bovine papillomavirus E2 protein.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1826-30 PMID: 2831538
  11. Specific binding of estrogen receptor to the estrogen response element.
    Mol Cell Biol. 1989 Jan;9(1):43-9 PMID: 2927397
  12. An altered DNA conformation in origin region I is a determinant for the binding of SV40 large T antigen.
    Cell. 1986 Mar 14;44(5):719-25 PMID: 3004744
  13. The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding domain.
    Nature. 1988 Aug 11;334(6182):543-6 PMID: 3043231
  14. Receptors bound to antiprogestin from abortive complexes with hormone responsive elements.
    Nature. 1988 Dec 15;336(6200):695-8 PMID: 3200320
  15. The Xenopus laevis estrogen receptor: sequence homology with human and avian receptors and identification of multiple estrogen receptor messenger ribonucleic acids.
    Mol Endocrinol. 1987 May;1(5):355-62 PMID: 3274894
  16. A single domain of the estrogen receptor confers deoxyribonucleic acid binding and transcriptional activation of the rat prolactin gene.
    Mol Endocrinol. 1988 Jan;2(1):14-21 PMID: 3398840
  17. Glucocorticoids are required for establishment and maintenance of an alteration in chromatin structure: induction leads to a reversible disruption of nucleosomes over an enhancer.
    EMBO J. 1991 Sep;10(9):2569-76 PMID: 1678348
  18. Purified estrogen receptor DNA binding domain expressed in Escherichia coli activates transcription of an estrogen-responsive promoter in cultured cells.
    J Biol Chem. 1991 Dec 15;266(35):24070-6 PMID: 1748680
  19. Fos-Jun heterodimers and Jun homodimers bend DNA in opposite orientations: implications for transcription factor cooperativity.
    Cell. 1991 Jul 26;66(2):317-26 PMID: 1906785
  20. POU proteins bend DNA via the POU-specific domain.
    EMBO J. 1991 Oct;10(10):3007-14 PMID: 1915275
  21. The progesterone receptor stimulates cell-free transcription by enhancing the formation of a stable preinitiation complex.
    Cell. 1990 Jan 26;60(2):247-57 PMID: 2153462
  22. An estrogen-responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells.
    Cell. 1986 Sep 26;46(7):1053-61 PMID: 3463433
  23. Glucocorticoid receptor mutants that define a small region sufficient for enhancer activation.
    Science. 1987 Apr 24;236(4800):423-7 PMID: 3563519
  24. Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
    Nature. 1986 Mar 13-19;320(6058):134-9 PMID: 3754034
  25. Sequence homologies in the region preceding the transcription initiation site of the liver estrogen-responsive vitellogenin and apo-VLDLII genes.
    Nucleic Acids Res. 1984 Nov 26;12(22):8611-26 PMID: 6504705
  26. A DNA curvature can substitute phage phi 29 regulatory protein p4 when acting as a transcriptional repressor.
    EMBO J. 1991 Nov;10(11):3429-38 PMID: 1655421
  27. Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA.
    Nature. 1991 Aug 8;352(6335):497-505 PMID: 1865905
  28. Solution structure of the glucocorticoid receptor DNA-binding domain.
    Science. 1990 Jul 13;249(4965):157-60 PMID: 2115209
  29. Hormonal induction of transfected genes depends on DNA topology.
    Mol Cell Biol. 1990 Feb;10(2):625-33 PMID: 2153920
  30. The NF-kappa B transcription factor induces DNA bending which is modulated by its 65-kD subunit.
    Nucleic Acids Res. 1990 Nov 25;18(22):6497-502 PMID: 2174540
  31. Solution structure of the DNA-binding domain of the oestrogen receptor.
    Nature. 1990 Nov 29;348(6300):458-61 PMID: 2247153
  32. Regulation of gene expression in vivo by liposome-mediated delivery of a purified transcription factor.
    J Biol Chem. 1990 Jun 25;265(18):10189-92 PMID: 2354996
  33. In vitro transcription enhancement by purified derivatives of the glucocorticoid receptor.
    Science. 1989 Jul 21;245(4915):298-301 PMID: 2473529
  34. Cooperative binding of estrogen receptor to imperfect estrogen-responsive DNA elements correlates with their synergistic hormone-dependent enhancer activity.
    EMBO J. 1989 Dec 1;8(12):3781-91 PMID: 2583118
  35. Asymmetric DNA bending induced by the yeast multifunctional factor TUF.
    J Biol Chem. 1989 May 25;264(15):8463-6 PMID: 2656680
  36. DNA bending by negative regulatory proteins: Gal and Lac repressors.
    Genes Dev. 1989 May;3(5):606-11 PMID: 2744457
  37. Empirical estimation of protein-induced DNA bending angles: applications to lambda site-specific recombination complexes.
    Nucleic Acids Res. 1988 Oct 25;16(20):9687-705 PMID: 2972993
  38. Bending of promoter DNA on binding of heat shock transcription factor.
    Nature. 1986 Oct 2-8;323(6087):459-61 PMID: 3020432
  39. Many transcription factors interact synergistically with steroid receptors.
    Science. 1988 Dec 9;242(4884):1418-20 PMID: 3201230
  40. The steroid and thyroid hormone receptor superfamily.
    Science. 1988 May 13;240(4854):889-95 PMID: 3283939
  41. Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
    Nucleic Acids Res. 1986 Nov 25;14(22):8755-70 PMID: 3466137
  42. Sequence and expression of human estrogen receptor complementary DNA.
    Science. 1986 Mar 7;231(4742):1150-4 PMID: 3753802
  43. Molecular characterization of the GCN4-DNA complex.
    Proc Natl Acad Sci U S A. 1990 Aug;87(16):6034-8 PMID: 2201019
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.5.2037-2042.1992
Published
1992-05-00
Pages
2037-42
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364374
Subset
IM
Grants
NICHD NIH HHS · HD-16720 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.5.2037-2042.1992 PMC Full Text View journal details
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