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PMID: 15673164 Published · ppublish English Comparative Study Journal Article

Native versus recombinant high-mobility group B1 proteins: functional activity in vitro.

Inflammation ·Vol. 28 ·No. 4 ·2004-08-00 ·Pages 221-9

Zimmermann K, Völkel D, Pable S, Lindner T, Kramberger F, Bahrami S, Scheiflinger F

Abstract

To compare the functional activity of native HMGB1 proteins from eukaryotic sources with HMGB1 from prokaryotic sources the cDNAs of human and murine HMGB1 were cloned and the proteins expressed in bacteria. Tissue-derived HMGB1 from calf thymus and HMGB1 secreted from Chinese hamster ovary (CHO) cells were purified. Human whole blood, THP-1 cells, and NIH/3T3 cells were exposed to HMGB1 proteins and the induction of tumor necrosis factor-alpha (TNF-alpha) release in whole blood and monocytic THP-1 cells and a proliferation assay in NIH/3T3 cells were used to study functional activity of HMGB1s in vitro. Native and recombinant HMGB1s induced TNF-alpha release in human blood and in THP-1 cells dose-dependently, but recombinant HMGB1s were more effective. Cell proliferation was induced by native and recombinant HMGB1s. The native HMGB1 proteins from eukaryotic sources exert the same (though less pronounced) biological activity in vitro as recombinant HMGB1 proteins from prokaryotic sources.

MeSH Terms
Amino Acid Sequence Animals CHO Cells Cattle Cell Line, Tumor Cell Proliferation Cricetinae Genetic Vectors HMGB1 Protein/antagonists & inhibitors,isolation & purification,physiology Humans Mice Molecular Sequence Data NIH 3T3 Cells Pyruvates/pharmacology Recombinant Fusion Proteins/genetics,isolation & purification,pharmacology Recombinant Proteins/genetics,isolation & purification,pharmacology Tumor Necrosis Factor-alpha/antagonists & inhibitors,metabolism
Chemicals
HMGB1 Protein Pyruvates Recombinant Fusion Proteins Recombinant Proteins Tumor Necrosis Factor-alpha ethyl pyruvate
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zimmermann Klaus
Baxter BioScience, Industriestrasse 72, 1220 Vienna, Austria.
Völkel Dirk
Pable Sabrina
Lindner Thomas
Kramberger Franziska
Bahrami Soheyl
Scheiflinger Friedrich
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Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1023/b:ifla.0000049047.61014.e3
Published
2004-08-00
Pages
221-9
Language
English
Region
United States
NLM ID
7600105
Subset
IM
External Links
PubMed source DOI: 10.1023/b:ifla.0000049047.61014.e3 View journal details
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