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PMID: 8947495 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Extracellular high-mobility group 1 protein is essential for murine erythroleukaemia cell differentiation.

The Biochemical journal ·Vol. 320 ( Pt 1) ·1996-11-15 ·Pages 253-6

Sparatore B, Passalacqua M, Patrone M, Melloni E, Pontremoli S

Abstract

A high-mobility group 1 (HMG1) protein type isolated from murine erythroleukaemia (MEL) cells promotes acceleration of the differentiation process when added to a MEL cell culture together with the inducer hexamethylene bisacetamide. We now provide direct evidence that the presence of HMG1 protein in the extracellular medium is essential for terminal erythroid differentiation. An extracellular function for HMG1 protein in MEL cell is further supported by a demonstration that this protein is released from MEL cells exposed to the chemical inducer and that the addition of an anti-(HMG1 protein) monoclonal antibody to the cell culture inhibits the differentiation process almost completely. The release of HMG1 protein from MEL cells is modulated by compounds affecting cell calcium homoeostasis, such as a calcium ionophore or verapamil. In fact, in the presence of the ionophore an increased rate of differentiation is accompanied by an enhanced extracellular release of HMG1 protein, whereas in the presence of verapamil both phenomena are significantly decreased.

MeSH Terms
Acetamides/pharmacology Animals Antineoplastic Agents/pharmacology Calcimycin/pharmacology Cell Differentiation/drug effects,physiology High Mobility Group Proteins/physiology Leukemia, Erythroblastic, Acute/pathology Mice Tumor Cells, Cultured
Chemicals
Acetamides Antineoplastic Agents High Mobility Group Proteins Calcimycin hexamethylene bisacetamide
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sparatore B
Institute of Biological Chemistry, University of Genova, Italy.
Passalacqua M
Patrone M
Melloni E
Pontremoli S
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3200253
Published
1996-11-15
Pages
253-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1217925
Subset
IM
External Links
PubMed source DOI: 10.1042/bj3200253 PMC Full Text View journal details
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