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PMID: 15574597 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromatin remodeling and histone modification in the conversion of oligodendrocyte precursors to neural stem cells.

Genes & development ·Vol. 18 ·No. 23 ·2004-12-01 ·Pages 2963-72

Kondo T, Raff M

Abstract

We showed previously that purified rat oligodendrocyte precursor cells (OPCs) can be induced by extracellular signals to convert to multipotent neural stem-like cells (NSLCs), which can then generate both neurons and glial cells. Because the conversion of precursor cells to stem-like cells is of both intellectual and practical interest, it is important to understand its molecular basis. We show here that the conversion of OPCs to NSLCs depends on the reactivation of the sox2 gene, which in turn depends on the recruitment of the tumor suppressor protein Brca1 and the chromatin-remodeling protein Brahma (Brm) to an enhancer in the sox2 promoter. Moreover, we show that the conversion is associated with the modification of Lys 4 and Lys 9 of histone H3 at the same enhancer. Our findings suggest that the conversion of OPCs to NSLCs depends on progressive chromatin remodeling, mediated in part by Brca1 and Brm.

MeSH Terms
Animals Base Sequence Cell Differentiation Cell Proliferation Chromatin/metabolism DNA Primers Histones/metabolism Immunohistochemistry Immunoprecipitation Oligodendroglia/cytology RNA, Messenger/genetics Rats Reverse Transcriptase Polymerase Chain Reaction Stem Cells/cytology
Chemicals
Chromatin DNA Primers Histones RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kondo Toru
Medical Research Council Laboratory for Molecular Cell Biology, Cell Biology Unit, University College London, London WC1E 6BT, United Kingdom. tk294@cam.ac.uk
Raff Martin
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.309404
Email
tk294@cam.ac.uk
Published
2004-12-01
Pages
2963-72
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC534656
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.309404 PMC Full Text View journal details
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