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PMID: 15520293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle.

Genome research ·Vol. 14 ·No. 11 ·2004-11-00 ·Pages 2308-18

Le Roch KG, Johnson JR, Florens L, Zhou Y, Santrosyan A, Grainger M, Yan SF, Williamson KC, Holder AA, Carucci DJ, Yates JR, Winzeler EA

Abstract

To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.

MeSH Terms
Animals Gene Expression Regulation/genetics Genes, Protozoan Life Cycle Stages/genetics Plasmodium falciparum/genetics,growth & development,metabolism Protozoan Proteins/biosynthesis RNA, Messenger/biosynthesis,genetics RNA, Protozoan/biosynthesis,genetics Transcription, Genetic/genetics
Chemicals
Protozoan Proteins RNA, Messenger RNA, Protozoan
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Le Roch Karine G
Department of Cell Biology ICND202, the Scripps Research Institute, La Jolla, California 92037, USA.
Johnson Jeffrey R
Florens Laurence
Zhou Yingyao
Santrosyan Andrey
Grainger Munira
Yan S Frank
Williamson Kim C
Holder Anthony A
Carucci Daniel J
Yates John R
Winzeler Elizabeth A
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.2523904
Published
2004-11-00
Pages
2308-18
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC525690
Subset
IM
Grants
NCRR NIH HHS · M01 RR000833 · United States
Medical Research Council · MC_U117532067 · United Kingdom
NCRR NIH HHS · M01 RR00833 · United States
External Links
PubMed source DOI: 10.1101/gr.2523904 PMC Full Text View journal details
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