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PMID: 15514020 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Centromere-encoded RNAs are integral components of the maize kinetochore.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 45 ·2004-11-09 ·Pages 15986-91

Topp CN, Zhong CX, Dawe RK

Abstract

RNA is involved in a variety of chromatin modification events, ranging from large-scale structural rearrangements to subtle local affects. Here, we extend the evidence for RNA-chromatin interactions to the centromere core. The data indicate that maize centromeric retrotransposons (CRMs) and satellite repeats (CentC) are not only transcribed, but that nearly half of the CRM and CentC RNA is tightly bound to centromeric histone H3 (CENH3), a key inner kinetochore protein. RNAs from another tandem repeat (180-bp knob sequence) or an abundant euchromatic retroelement (Opie) are undetectable within the same anti-CENH3 immune complexes. Both sense and antisense strands of CRM and CentC, but not small interfering RNAs homologous to either repeat, were found to coimmunoprecipitate with CENH3. The bulk of the immunoprecipitated RNA ranged in size from 40 to 200 nt. These data provide evidence for a pool of protected, single-stranded centromeric RNA within the centromere/kinetochore complex.

MeSH Terms
Base Sequence Centromere/genetics,metabolism DNA, Plant/genetics Histones/metabolism Kinetochores/metabolism Plant Proteins/metabolism RNA, Plant/genetics Retroelements/genetics Zea mays/genetics,metabolism
Chemicals
DNA, Plant Histones Plant Proteins RNA, Plant Retroelements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Topp Christopher N
Departments of Plant Biology and Genetics, University of Georgia, Athens, GA 30602, USA.
Zhong Cathy X
Dawe R Kelly
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0407154101
Published
2004-11-09
Epub
2004-00-28
Pages
15986-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC528775
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0407154101 PMC Full Text View journal details
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