A torrid zone on mouse chromosome 1 containing a cluster of recombinational hotspots.

Genetics ·Vol. 169 ·No. 2 ·2005-02-00 ·Pages 833-41

Kelmenson PM, Petkov P, Wang X, Higgins DC, Paigen BJ, Paigen K

Abstract

Within the 2.38-Mb Ath1 region of mouse chromosome 1, 42 of 45 genetic crossovers from crosses between C57BL/6J (B6) and either C3H/HeJ (H) or Mus spretus (SPRET) occurred in four zones (A-D); zone A, 100 kb long, contained a cluster of at least four recombination hotspots. F1 sperm assays indicate that within this "torrid zone" the most active hotspot (A3) can initiate recombination on H and SPRET but not B6 chromosomes. The A3 DNA sequence contains a (G/C)TTT repeat, long stretches of A or T, and a cyclic variation in AT content. Recombination was drastically reduced in a cross between B6 and a B6.SPRET Ath1 congenic strain, but was unaffected in a B6 x B6.H Ath1 congenic cross. Similar nonrandom clustering of hotspots has been observed in yeast and the major histocompatibility complexes of human and mouse. To the extent that torrid zones are a general feature of mammalian genomes, they have considerable implications for genetic mapping strategies in both human populations and mouse crosses.

MeSH Terms

Animals Base Sequence Chromosome Mapping Chromosomes Crosses, Genetic Male Mice Mice, Congenic Mice, Inbred C3H Mice, Inbred C57BL Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Single Nucleotide Recombination, Genetic Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA Species Specificity Spermatozoa/cytology

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kelmenson Peter M

The Jackson Laboratory, Bar Harbor, Maine 04609-1500, USA.

Petkov Petko

Wang Xiaosong

Higgins David C

Paigen Beverly J

Paigen Kenneth

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Article Info

Journal

Genetics

Abbr.

Genetics

ISSN

0016-6731

DOI

10.1534/genetics.104.035063

Published

2005-02-00

Epub

2004-00-16

Pages

833-41

Language

English

Region

United States

NLM ID

0374636

PMCID

PMC1449111

Subset

External Links

PubMed source DOI: 10.1534/genetics.104.035063 PMC Full Text View journal details

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A torrid zone on mouse chromosome 1 containing a cluster of recombinational hotspots.

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