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PMID: 15486206 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

DNA repair by a Rad22-Mus81-dependent pathway that is independent of Rhp51.

Nucleic acids research ·Vol. 32 ·No. 18 ·2004-00-00 ·Pages 5570-81

Doe CL, Osman F, Dixon J, Whitby MC

Abstract

In budding yeast most Rad51-dependent and -independent recombination depends on Rad52. In contrast, its homologue in fission yeast, Rad22, was assumed to play a less critical role possibly due to functional redundancy with another Rad52-like protein Rti1. We show here that this is not the case. Rad22 like Rad52 plays a central role in recombination being required for both Rhp51-dependent and -independent events. Having established this we proceed to investigate the involvement of the Mus81-Eme1 endonuclease in these pathways. Mus81 plays a relatively minor role in the Rhp51-dependent repair of DNA damage induced by ultraviolet light. In contrast Mus81 has a key role in the Rad22-dependent (Rhp51-independent) repair of damage induced by camptothecin, hydroxyurea and methyl-methanesulfonate. Furthermore, spontaneous intrachromosomal recombination that gives rise to deletion recombinants is impaired in a mus81 mutant. From these data we propose that a Rad22-Mus81-dependent (Rhp51-independent) pathway is an important mechanism for the repair of DNA damage in fission yeast. Consistent with this we show that in vitro Rad22 can promote strand invasion to form a D-loop that can be cleaved by Mus81.

MeSH Terms
DNA Repair DNA, Fungal/chemistry,genetics,metabolism DNA-Binding Proteins/deficiency,genetics,metabolism Endonucleases/deficiency,genetics,metabolism Models, Genetic Mutation/genetics Nucleic Acid Conformation Rad51 Recombinase Recombination, Genetic/genetics Repetitive Sequences, Nucleic Acid/genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces/genetics,metabolism Schizosaccharomyces pombe Proteins/genetics,metabolism
Chemicals
DNA, Fungal DNA-Binding Proteins MUS81 protein, S pombe RHP51 protein, S pombe Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins rad52 protein, S pombe Rad51 Recombinase Eme1 protein, S pombe Endonucleases MUS81 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Doe Claudette L
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Osman Fekret
Dixon Julie
Whitby Matthew C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkh853
Published
2004-00-00
Epub
2004-00-14
Pages
5570-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC524275
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkh853 PMC Full Text View journal details
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