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PMID: 1547773 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The E.coli fis promoter is subject to stringent control and autoregulation.

The EMBO journal ·Vol. 11 ·No. 3 ·1992-03-00 ·Pages 1075-83

Ninnemann O, Koch C, Kahmann R

Abstract

The DNA binding protein FIS is involved in processes like site specific DNA inversion, lambda excision and stimulation of stable RNA synthesis in Escherichia coli. The amount of FIS protein is subject to dramatic changes during growth. We demonstrate that fis is part of an operon with one ORF of unknown function preceding the fis gene. Regulation of fis synthesis occurs at the transcriptional level. Within 15 min after nutritional upshift a large burst of fis mRNA is produced which levels off when cells begin to grow. By mutational analysis using promoter-lacZ fusions we demonstrate that the fis promoter is autoregulated by FIS. Growth phase regulation of the fis promoter depends on the presence of a GC motif downstream of the -10 region. We show that the fis promoter is subject to stringent control and discuss this unusual feature with respect to the known and putative functions FIS serves in E. coli.

Related Genes
fis lacZ
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Carrier Proteins/genetics DNA Fingerprinting DNA, Bacterial/genetics DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics Escherichia coli Proteins Factor For Inversion Stimulation Protein Gene Expression Regulation, Bacterial Genes, Bacterial Integration Host Factors Molecular Sequence Data Operon Plasmids Promoter Regions, Genetic RNA, Messenger/biosynthesis Transcription, Genetic beta-Galactosidase/metabolism
Chemicals
Carrier Proteins DNA, Bacterial DNA-Binding Proteins Escherichia coli Proteins Factor For Inversion Stimulation Protein Integration Host Factors RNA, Messenger integration host factor, E coli beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ninnemann O
Institut für Genbiologische Forschung Berlin GmbH, Germany.
Koch C
Kahmann R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-03-00
Pages
1075-83
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556548
Subset
IM
Databases
GENBANK
L12698, L12699, L12700, L12701, L12702, L12703, X62399, X65069, X65070, Y00044
External Links
PubMed source PMC Full Text View journal details
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