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PMID: 1545826 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential expression of oocyte-type class III genes with fraction TFIIIC from immature or mature oocytes.

Molecular and cellular biology ·Vol. 12 ·No. 3 ·1992-03-00 ·Pages 946-53

Reynolds WF, Johnson DL

Abstract

The Xenopus OAX genes can be expressed in oocytes but are virtually inactive in somatic tissues. The tRNA(Met1) (tMET) genes also appear to be developmentally regulated. We have examined the reason for the differential expression of these class III genes. Analysis of the transcriptional activities of extracts derived from immature and mature oocytes revealed that the developmental regulation of these genes can be reproduced in vitro. We have partially purified the required transcription factors B and C from these extracts to ascertain the components responsible for this differential activity. The immature oocyte C fraction activates the tMET and OAX genes when reconstituted with either the immature or mature oocyte-derived B fraction. In contrast, the mature oocyte C fraction fails to activate these genes regardless of which B fraction is used. Both C fractions activated the somatic 5S gene. Purification of the oocyte C fractions by phosphocellulose or B box DNA affinity chromatography failed to separate additional activities responsible for the differential expression of OAX or tMET. By using template exclusion assays, the inability of the mature oocyte C fraction to activate transcription was correlated with an inability to form stable transcription complexes with the tMET or OAX gene.

Related Genes
OAX
MeSH Terms
Animals Base Sequence Cell Fractionation Chromatography, Affinity DNA Gene Expression Regulation Molecular Sequence Data Multigene Family Oocytes/growth & development,metabolism RNA, Transfer, Met/genetics,metabolism Transcription Factors/metabolism Transcription Factors, TFIII Transcription, Genetic Xenopus laevis
Chemicals
RNA, Transfer, Met Transcription Factors Transcription Factors, TFIII transcription factor TFIIIC DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reynolds W F
La Jolla Cancer Research Foundation, California 92037.
Johnson D L
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58 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.3.946-953.1992
Published
1992-03-00
Pages
946-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369526
Subset
IM
Grants
NIGMS NIH HHS · GM34888 · United States
External Links
PubMed source DOI: 10.1128/mcb.12.3.946-953.1992 PMC Full Text View journal details
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