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PMID: 3375059 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence differences upstream of the promoters are involved in the differential expression of the Xenopus somatic and oocyte 5S RNA genes.

Nucleic acids research ·Vol. 16 ·No. 8 ·1988-04-25 ·Pages 3391-403

Reynolds WF, Azer K

Abstract

The Xenopus somatic and oocyte 5S RNA genes are differentially expressed in extracts of whole oocytes. In such extracts, sequence differences preceding the internal promoters significantly alter the relative activities of these genes. Following exchange of the sequences preceding the promoter, the activity of the somatic 5S gene decreased and that of the oocyte 5S gene increased. As a result, a 100 fold somatic transcriptional advantage was reduced to 5 fold. Analysis of deletion mutants showed that the relevant sequence differences are located between -34 and +37 relative to the initiation site. The observed transcriptional modulation is due both to sequence differences 5' to the initiation site and at positions 30 and 37 within the coding region.

MeSH Terms
Animals Base Sequence Gene Expression Regulation Molecular Sequence Data Oocytes Organ Specificity Promoter Regions, Genetic RNA, Ribosomal/genetics RNA, Ribosomal, 5S/genetics Regulatory Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transcription, Genetic Xenopus laevis/genetics
Chemicals
RNA, Ribosomal RNA, Ribosomal, 5S
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Reynolds W F
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Azer K
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.8.3391
Published
1988-04-25
Pages
3391-403
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336501
Subset
IM
Grants
NIGMS NIH HHS · GM34888 · United States
External Links
PubMed source DOI: 10.1093/nar/16.8.3391 PMC Full Text View journal details
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