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PMID: 1535575 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression and ligand-binding function of the integrin alpha 4 beta 1 (VLA-4) on neural-crest-derived tumor cell lines.

Clinical & experimental metastasis ·Vol. 10 ·No. 4 ·1992-07-00 ·Pages 281-90

Bednarczyk JL, McIntyre BW

Abstract

Human neural-crest-derived tumor cell lines, including three neuroblastomas, an astrocytoma, a glioblastoma, a rhabdomyosarcoma and a melanoma were screened for the expression of the integrin alpha 4 beta 1 (VLA-4). The neuroblastomas IMR-32 and SK-N-SH, the astrocytoma 131-INI, the glioblastoma Fogerty and the rhabdomyosarcoma TE-671 expressed alpha 4 beta 1 as determined by cytofluorometry and immunoprecipitation. Another neuroblastoma line, LA-N-1, did not express alpha 4 beta 1. Analysis of immunoprecipitated alpha 4 beta 1 showed that the alpha 4 subunit from the various cell types differed in relative molecular weight (M(r)). The variability in the observed M(r) could be accounted for by differences in the levels of N-linked glycosylation. The observed variability in M(r) did not appear to affect function since intact cells and solubilized alpha 4 beta 1 bound to a synthetic peptide identical in sequence to the CS-1 region of the alternatively spliced IIICS domain of fibronectin, a known alpha 4 beta 1 ligand.

MeSH Terms
Amino Acid Sequence Astrocytoma/metabolism,physiopathology,ultrastructure Chromatography, Affinity Glioma/metabolism,physiopathology,ultrastructure Glycosylation Humans Integrin alpha4beta1 Integrins/metabolism,physiology Melanoma/metabolism,physiopathology,ultrastructure Molecular Sequence Data Nervous System Neoplasms/metabolism,physiopathology,ultrastructure Neural Crest/pathology Neuroblastoma/metabolism,physiopathology,ultrastructure Precipitin Tests Receptors, Fibronectin Receptors, Immunologic/physiology Rhabdomyosarcoma/metabolism,physiopathology,ultrastructure Tumor Cells, Cultured
Chemicals
Integrin alpha4beta1 Integrins Receptors, Fibronectin Receptors, Immunologic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bednarczyk J L
Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
McIntyre B W
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Article Info
Journal
Clinical & experimental metastasis
Abbr.
Clin Exp Metastasis
ISSN
0262-0898
DOI
10.1007/BF00133564
Published
1992-07-00
Pages
281-90
Language
English
Region
Netherlands
NLM ID
8409970
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00133564 View journal details
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