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PMID: 2022922 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human lung tumor-associated antigen identified as an extracellular matrix adhesion molecule.

The Journal of experimental medicine ·Vol. 173 ·No. 5 ·1991-05-01 ·Pages 1111-9

Chen FA, Repasky EA, Bankert RB

Abstract

A single chain glycoprotein with an estimated molecular mass of 160 kD (gp160) was previously identified as a human lung tumor-associated antigen. This tumor marker is shown here to be associated noncovalently with a second 130-kD protein. Sequential immunoprecipitation studies of surface iodinated lung tumor cell lysates reveal that this heterodimeric complex is indistinguishable serologically and structurally from the integrin VLA-2, found originally on activated T lymphocytes and platelets. The VLA-2-like complex expressed on the lung tumors possesses similar characteristic Mg2+ dependent binding of collagen and laminin as observed with VLA-2 on normal cells. RNA analysis indicates that human lung tumors express at least 20 times more VLA-2 alpha chain message than normal adult human lung tissue. The results presented here raise the possibility that the overproduction of VLA-2 may be involved in the pathogenesis of human lung tumors by modulating the invasive and metastatic potential of the tumor.

MeSH Terms
Antigens, Tumor-Associated, Carbohydrate/analysis,genetics,metabolism Biomarkers, Tumor/analysis Blotting, Northern Cell Adhesion Molecules/analysis,genetics,metabolism Collagen/metabolism Electrophoresis, Polyacrylamide Gel Extracellular Matrix Proteins/chemistry,metabolism Gene Expression Regulation, Neoplastic/physiology Humans Laminin/metabolism Lung Neoplasms/etiology,metabolism,pathology Neoplasm Metastasis/physiopathology Precipitin Tests Receptors, Very Late Antigen/analysis,genetics,metabolism Tumor Cells, Cultured/metabolism,pathology,ultrastructure
Chemicals
Antigens, Tumor-Associated, Carbohydrate Biomarkers, Tumor Cell Adhesion Molecules Extracellular Matrix Proteins Laminin Receptors, Very Late Antigen Collagen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chen F A
Roswell Park Cancer Institute, (Unit of New York State Department of Health), Department of Molecular Immunology, Buffalo, New York 14263.
Repasky E A
Bankert R B
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.173.5.1111
Published
1991-05-01
Pages
1111-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118860
Subset
IM
Grants
NCI NIH HHS · CA-22786 · United States
NCI NIH HHS · CA-25253 · United States
NCI NIH HHS · CA-33462 · United States
External Links
PubMed source DOI: 10.1084/jem.173.5.1111 PMC Full Text View journal details
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