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PMID: 15342533 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Stochastic and epigenetic changes of gene expression in Arabidopsis polyploids.

Genetics ·Vol. 167 ·No. 4 ·2004-08-00 ·Pages 1961-73

Wang J, Tian L, Madlung A, Lee HS, Chen M, Lee JJ, Watson B, Kagochi T, Comai L, Chen ZJ

Abstract

Polyploidization is an abrupt speciation mechanism for eukaryotes and is especially common in plants. However, little is known about patterns and mechanisms of gene regulation during early stages of polyploid formation. Here we analyzed differential expression patterns of the progenitors' genes among successive selfing generations and independent lineages. The synthetic Arabidopsis allotetraploid lines were produced by a genetic cross between A. thaliana and A. arenosa autotetraploids. We found that some progenitors' genes are differentially expressed in early generations, whereas other genes are silenced in late generations or among different siblings within a selfing generation, suggesting that the silencing of progenitors' genes is rapidly and/or stochastically established. Moreover, a subset of genes is affected in autotetraploid and multiple independent allotetraploid lines and in A. suecica, a natural allotetraploid derived from A. thaliana and A. arenosa, indicating locus-specific susceptibility to ploidy-dependent gene regulation. The role of DNA methylation in silencing progenitors' genes is tested in DNA-hypomethylation transgenic lines of A. suecica using RNA interference (RNAi). Two silenced genes are reactivated in both ddm1- and met1-RNAi lines, consistent with the demethylation of centromeric repeats and gene-specific regions in the genome. A rapid and stochastic process of differential gene expression is reinforced by epigenetic regulation during polyploid formation and evolution.

MeSH Terms
Arabidopsis/genetics Arabidopsis Proteins/genetics Base Sequence Cloning, Molecular DNA Primers Gene Expression Regulation, Plant/genetics Gene Silencing Polymorphism, Restriction Fragment Length Polyploidy RNA, Plant/genetics RNA, Small Interfering/genetics Stochastic Processes
Chemicals
Arabidopsis Proteins DNA Primers RNA, Plant RNA, Small Interfering
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wang Jianlin
Genetics Program, Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843-2474, USA.
Tian Lu
Madlung Andreas
Lee Hyeon-Se
Chen Meng
Lee Jinsuk J
Watson Brian
Kagochi Trevor
Comai Luca
Chen Z Jeffrey
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.027896
Published
2004-08-00
Pages
1961-73
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1471021
Subset
IM
Grants
NIGMS NIH HHS · R01 GM067015 · United States
NIGMS NIH HHS · GM67015 · United States
External Links
PubMed source DOI: 10.1534/genetics.104.027896 PMC Full Text View journal details
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