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PMID: 15342494 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Arabidopsis COP10 forms a complex with DDB1 and DET1 in vivo and enhances the activity of ubiquitin conjugating enzymes.

Genes & development ·Vol. 18 ·No. 17 ·2004-09-01 ·Pages 2172-81

Yanagawa Y, Sullivan JA, Komatsu S, Gusmaroli G, Suzuki G, Yin J, Ishibashi T, Saijo Y, Rubio V, Kimura S, Wang J, Deng XW

Abstract

COP10 is a ubiquitin-conjugating enzyme variant (UEV), which is thought to act together with COP1, DET1, and the COP9 signalosome (CSN) in Arabidopsis to repress photomorphogenesis. Here, we demonstrate that COP10 interacts with ubiquitin-conjugating enzymes (E2s) in vivo, and can enhance their activity in vitro, an activity distinct from previous characterized UEVs such as MMS2 and UEV1. Furthermore, we show that COP10 forms a complex with UV-damaged DNA-binding protein 1a (DDB1a) and de-etiolated 1 (DET1), and physically interacts with COP1 and the CSN. Purified CDD (COP10, DDB1, DET1) complex also shows enhancement of E2 activity (UEA) similar to that observed with COP10 itself. Our data suggests that COP10, along with COP1 and the CSN, promotes the degradation of positive regulators of photomorphogenesis, such as the transcription factor HY5, via the ubiquitin/26S proteasome system. Thus, the CDD complex may act as a ubiquitylation-promoting factor to regulate photomorphogenesis.

MeSH Terms
Arabidopsis/growth & development,metabolism Arabidopsis Proteins/metabolism COP9 Signalosome Complex Chromatography, Gel DNA Primers DNA-Binding Proteins/metabolism Immunoblotting Intracellular Signaling Peptides and Proteins Light Models, Biological Morphogenesis Nuclear Proteins/metabolism Plants, Genetically Modified Precipitin Tests Proteins/metabolism Ubiquitin/metabolism Ubiquitin-Conjugating Enzymes/metabolism Ubiquitin-Protein Ligases
Chemicals
Arabidopsis Proteins CSN1 protein, Arabidopsis DET1 protein, Arabidopsis DNA Primers DNA-Binding Proteins Intracellular Signaling Peptides and Proteins Nuclear Proteins Proteins Ubiquitin COP10 protein, Arabidopsis Ubiquitin-Conjugating Enzymes AT2G32950 protein, Arabidopsis Ubiquitin-Protein Ligases COP9 Signalosome Complex
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Yanagawa Yuki
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104, USA.
Sullivan James A
Komatsu Setsuko
Gusmaroli Giuliana
Suzuki Genki
Yin Jianning
Ishibashi Toyotaka
Saijo Yusuke
Rubio Vicente
Kimura Seisuke
Wang Jian
Deng Xing Wang
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1229504
Published
2004-09-01
Pages
2172-81
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC515294
Subset
IM
Grants
NIGMS NIH HHS · R01 GM047850 · United States
NIGMS NIH HHS · R37 GM047850 · United States
NIGMS NIH HHS · GM-47850 · United States
External Links
PubMed source DOI: 10.1101/gad.1229504 PMC Full Text View journal details
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