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PMID: 14597662 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The COP1-SPA1 interaction defines a critical step in phytochrome A-mediated regulation of HY5 activity.

Genes & development ·Vol. 17 ·No. 21 ·2003-11-01 ·Pages 2642-7

Saijo Y, Sullivan JA, Wang H, Yang J, Shen Y, Rubio V, Ma L, Hoecker U, Deng XW

Abstract

Arabidopsis COP1 is a constitutive repressor of photomorphogenesis that interacts with photomorphogenesis-promoting factors such as HY5 to promote their proteasome-mediated degradation. SPA1 is a repressor of phytochrome A-mediated responses to far-red light. Here we report that COP1 acts as part of a large protein complex and interacts with SPA1 in a light-dependent manner. We further demonstrate the E3 ubiquitin ligase activity of COP1 on HY5 in vitro and the alteration of that activity by SPA1. Thus, the COP1-SPA1 interaction defines a critical step in coordinating COP1-mediated ubiquitination and subsequent degradation of HY5 with PHYA signaling.

MeSH Terms
Arabidopsis/metabolism Arabidopsis Proteins/metabolism Basic-Leucine Zipper Transcription Factors Nuclear Proteins/metabolism Phytochrome/metabolism Phytochrome A Ubiquitin-Protein Ligases/metabolism
Chemicals
Arabidopsis Proteins Basic-Leucine Zipper Transcription Factors HY5 protein, Arabidopsis Nuclear Proteins PHYA protein, Arabidopsis Phytochrome A Phytochrome AT2G32950 protein, Arabidopsis Ubiquitin-Protein Ligases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Saijo Yusuke
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Sullivan James A
Wang Haiyang
Yang Jianping
Shen Yunping
Rubio Vicente
Ma Ligeng
Hoecker Ute
Deng Xing Wang
References (18)
18 references, click to expand
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1122903
Published
2003-11-01
Pages
2642-7
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC280614
Subset
IM
Grants
NIGMS NIH HHS · R01 GM047850 · United States
NIGMS NIH HHS · R37 GM047850 · United States
NIGMS NIH HHS · GM47850 · United States
External Links
PubMed source DOI: 10.1101/gad.1122903 PMC Full Text View journal details
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