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PMID: 11701877 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular characterization of subunit 6 of the COP9 signalosome and its role in multifaceted developmental processes in Arabidopsis.

The Plant cell ·Vol. 13 ·No. 11 ·2001-11-00 ·Pages 2393-407

Peng Z, Serino G, Deng XW

Abstract

The COP9 signalosome is a highly conserved protein complex initially identified as a repressor of photomorphogenesis. Here, we report that subunit 6 of the Arabidopsis COP9 signalosome is encoded by a family of two genes (CSN6A and CSN6B) located on chromosomes V and IV, respectively. The CSN6A and CSN6B proteins share 87% amino acid identity and contain a MPR1p and PAD1p N-terminal (MPN) domain at the N-terminal region. The CSN6 proteins share homology with CSN5 and belong to the Mov34 superfamily of proteins. CSN6 proteins present only in the complex form and coimmunoprecipitate with other known subunits of the COP9 signalosome. Partial loss-of-function strains of the COP9 signalosome created by antisense and cosuppression with CSN6A exhibit diverse developmental defects, including homeotic organ transformation, symmetric body organization, and organ boundary definition. Protein blot analysis revealed that the defective plants accumulate significant amounts of ubiquitinated proteins, supporting the conclusion that the COP9 signalosome regulates multifaceted developmental processes through its involvement in ubiquitin/proteasome-mediated protein degradation.

MeSH Terms
Amino Acid Sequence Arabidopsis/classification,genetics Arabidopsis Proteins COP9 Signalosome Complex Cloning, Molecular DNA, Complementary/genetics Humans Molecular Sequence Data Multigene Family Multiprotein Complexes Peptide Hydrolases Phenotype Phylogeny Plant Proteins/chemistry,genetics,metabolism Plants, Genetically Modified/genetics Protein Kinases/chemistry,genetics,metabolism Protein Subunits Proteins/genetics Saccharomyces cerevisiae/genetics Sequence Alignment Sequence Homology, Amino Acid Transformation, Genetic
Chemicals
Arabidopsis Proteins CSN6B protein, Arabidopsis DNA, Complementary Multiprotein Complexes Plant Proteins Protein Subunits Proteins Protein Kinases Peptide Hydrolases COP9 Signalosome Complex
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Peng Z
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8104, USA.
Serino G
Deng X W
References (50)
50 references, click to expand
  1. Arabidopsis FUSCA5 encodes a novel phosphoprotein that is a component of the COP9 complex.
    Plant Cell. 1999 May;11(5):839-48 PMID: 10330469
  2. Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIRI in mediating auxin response.
    Science. 2001 May 18;292(5520):1379-82 PMID: 11337587
  3. Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly.
    J Biol Chem. 1997 Oct 24;272(43):27042-52 PMID: 9341143
  4. A novel protein complex involved in signal transduction possessing similarities to 26S proteasome subunits.
    FASEB J. 1998 Apr;12(6):469-78 PMID: 9535219
  5. Making sense of the COP9 signalosome. A regulatory protein complex conserved from Arabidopsis to human.
    Trends Genet. 1999 Mar;15(3):98-103 PMID: 10203806
  6. The subunit 1 of the COP9 signalosome suppresses gene expression through its N-terminal domain and incorporates into the complex through the PCI domain.
    J Mol Biol. 2001 Jan 5;305(1):1-9 PMID: 11114242
  7. Arabidopsis homologs of a c-Jun coactivator are present both in monomeric form and in the COP9 complex, and their abundance is differentially affected by the pleiotropic cop/det/fus mutations.
    Plant Cell. 1998 Nov;10(11):1779-90 PMID: 9811788
  8. Degradation of the cyclin-dependent-kinase inhibitor p27Kip1 is instigated by Jab1.
    Nature. 1999 Mar 11;398(6723):160-5 PMID: 10086358
  9. Evidence for FUS6 as a component of the nuclear-localized COP9 complex in Arabidopsis.
    Plant Cell. 1996 Nov;8(11):2047-56 PMID: 8953769
  10. Unified nomenclature for the COP9 signalosome and its subunits: an essential regulator of development
    Trends Genet. 2000 Jul;16(7):289 PMID: 10858657
  11. A genetic screen for modifiers of UFO meristem activity identifies three novel FUSED FLORAL ORGANS genes required for early flower development in Arabidopsis.
    Genetics. 1998 Jun;149(2):579-95 PMID: 9611175
  12. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.
    Cell. 2000 Mar 31;101(1):25-33 PMID: 10778853
  13. c-Jun can recruit JNK to phosphorylate dimerization partners via specific docking interactions.
    Cell. 1996 Nov 29;87(5):929-39 PMID: 8945519
  14. A FUSCA gene of Arabidopsis encodes a novel protein essential for plant development.
    Plant Cell. 1994 Jan;6(1):25-41 PMID: 8130643
  15. Role of a COP1 interactive protein in mediating light-regulated gene expression in arabidopsis.
    Plant Cell. 1998 Jul;10(7):1083-94 PMID: 9668129
  16. The COP9 complex, a novel multisubunit nuclear regulator involved in light control of a plant developmental switch.
    Cell. 1996 Jul 12;86(1):115-21 PMID: 8689678
  17. The cellular level of PR500, a protein complex related to the 19S regulatory particle of the proteasome, is regulated in response to stresses in plants.
    Mol Biol Cell. 2001 Feb;12(2):383-92 PMID: 11179422
  18. The role of the COP/DET/FUS genes in light control of arabidopsis seedling development.
    Plant Physiol. 1996 Nov;112(3):871-8 PMID: 8938399
  19. The FUSCA genes of Arabidopsis: negative regulators of light responses.
    Mol Gen Genet. 1994 Aug 2;244(3):242-52 PMID: 8058035
  20. A yeast mating-selection scheme for detection of protein-protein interactions.
    Nucleic Acids Res. 1994 May 11;22(9):1778-9 PMID: 8202391
  21. COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system.
    EMBO J. 2001 Apr 2;20(7):1630-9 PMID: 11285227
  22. A LEAFY co-regulator encoded by UNUSUAL FLORAL ORGANS.
    Curr Biol. 1997 Feb 1;7(2):95-104 PMID: 9016705
  23. Leaf polarity and meristem formation in Arabidopsis.
    Development. 1998 Aug;125(15):2935-42 PMID: 9655815
  24. A complement of ten essential and pleiotropic arabidopsis COP/DET/FUS genes is necessary for repression of photomorphogenesis in darkness.
    Plant Physiol. 1996 Mar;110(3):731-42 PMID: 8819865
  25. HIV-1 Vpr interacts with a human 34-kDa mov34 homologue, a cellular factor linked to the G2/M phase transition of the mammalian cell cycle.
    Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3419-24 PMID: 9520381
  26. UNUSUAL FLORAL ORGANS Controls Meristem Identity and Organ Primordia Fate in Arabidopsis.
    Plant Cell. 1995 Sep;7(9):1485-1499 PMID: 12242408
  27. Electron microscopy and subunit-subunit interaction studies reveal a first architecture of COP9 signalosome.
    J Mol Biol. 2000 Jul 28;300(5):1169-78 PMID: 10903862
  28. Parallels between UNUSUAL FLORAL ORGANS and FIMBRIATA, genes controlling flower development in Arabidopsis and Antirrhinum.
    Plant Cell. 1995 Sep;7(9):1501-10 PMID: 8589630
  29. UFO: an Arabidopsis gene involved in both floral meristem and floral organ development.
    Plant Cell. 1995 May;7(5):529-48 PMID: 7780306
  30. The COP9 complex is conserved between plants and mammals and is related to the 26S proteasome regulatory complex.
    Curr Biol. 1998 Jul 30-Aug 13;8(16):919-22 PMID: 9707402
  31. Arabidopsis COP9 is a component of a novel signaling complex mediating light control of development.
    Cell. 1994 Jul 15;78(1):117-24 PMID: 8033203
  32. The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p.
    Genes Dev. 1998 Jan 15;12(2):198-207 PMID: 9436980
  33. A role of Arabidopsis COP9 signalosome in multifaceted developmental processes revealed by the characterization of its subunit 3.
    Development. 2001 Nov;128(21):4277-88 PMID: 11684663
  34. COP9: a new genetic locus involved in light-regulated development and gene expression in arabidopsis.
    Plant Cell. 1992 Dec;4(12):1507-18 PMID: 1467650
  35. Arabidopsis COP8, COP10, and COP11 genes are involved in repression of photomorphogenic development in darkness.
    Plant Cell. 1994 May;6(5):629-43 PMID: 8038603
  36. Targeted destabilization of HY5 during light-regulated development of Arabidopsis.
    Nature. 2000 May 25;405(6785):462-6 PMID: 10839542
  37. The COP9/signalosome complex is conserved in fission yeast and has a role in S phase.
    Curr Biol. 1999 Dec 2;9(23):1427-30 PMID: 10607571
  38. Degradation of Aux/IAA proteins is essential for normal auxin signalling.
    Plant J. 2000 Mar;21(6):553-62 PMID: 10758506
  39. The UNUSUAL FLORAL ORGANS gene of Arabidopsis thaliana is an F-box protein required for normal patterning and growth in the floral meristem.
    Plant J. 1999 Nov;20(4):433-45 PMID: 10607296
  40. Function of the ubiquitin-proteasome pathway in auxin response.
    Trends Biochem Sci. 2000 Mar;25(3):133-8 PMID: 10694884
  41. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2.
    Cell. 1993 Nov 19;75(4):791-803 PMID: 8242750
  42. Function of the apetala-1 gene during Arabidopsis floral development.
    Plant Cell. 1990 Aug;2(8):741-53 PMID: 1983792
  43. Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.
    Science. 2001 May 18;292(5520):1382-5 PMID: 11337588
  44. A new group of conserved coactivators that increase the specificity of AP-1 transcription factors.
    Nature. 1996 Oct 3;383(6599):453-7 PMID: 8837781
  45. Light modulation of vegetative development.
    Plant Cell. 1997 Jul;9(7):1225-34 PMID: 9254936
  46. Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction in yeast and mammalian cells.
    Mol Cell Biol. 1996 Dec;16(12):6698-706 PMID: 8943324
  47. Genetic analysis of organ fusion in Arabidopsis thaliana.
    Genetics. 1998 Jun;149(2):607-19 PMID: 9611177
  48. Arabidopsis cop8 and fus4 mutations define the same gene that encodes subunit 4 of the COP9 signalosome.
    Plant Cell. 1999 Oct;11(10):1967-80 PMID: 10521526
  49. p38JAB1 binds to the intracellular precursor of the lutropin/choriogonadotropin receptor and promotes its degradation.
    J Biol Chem. 2000 May 5;275(18):13386-93 PMID: 10788448
  50. The COP9 signalosome is essential for development of Drosophila melanogaster.
    Curr Biol. 1999 Oct 21;9(20):1187-90 PMID: 10531038
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010248
Published
2001-11-00
Pages
2393-407
Language
English
Region
England
NLM ID
9208688
PMCID
PMC139460
Subset
IM
Databases
GENBANK
AF434762, AY048692
External Links
PubMed source DOI: 10.1105/tpc.010248 PMC Full Text View journal details
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