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PMID: 15328537 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane.

PLoS biology ·Vol. 2 ·No. 9 ·2004-09-00 ·Pages E231

Holaska JM, Kowalski AK, Wilson KL

Abstract

X-linked Emery-Dreifuss muscular dystrophy is caused by loss of emerin, a LEM-domain protein of the nuclear inner membrane. To better understand emerin function, we used affinity chromatography to purify emerin-binding proteins from nuclear extracts of HeLa cells. Complexes that included actin, alphaII-spectrin and additional proteins, bound specifically to emerin. Actin polymerization assays in the presence or absence of gelsolin or capping protein showed that emerin binds and stabilizes the pointed end of actin filaments, increasing the actin polymerization rate 4- to 12-fold. We propose that emerin contributes to the formation of an actin-based cortical network at the nuclear inner membrane, conceptually analogous to the actin cortical network at the plasma membrane. Thus, in addition to disrupting transcription factors that bind emerin, loss of emerin may destabilize nuclear envelope architecture by weakening a nuclear actin network.

MeSH Terms
Actin Cytoskeleton/chemistry Actins/chemistry Binding Sites Cell Membrane/metabolism Cell Nucleus/metabolism Chromatography, Affinity Gelsolin/chemistry HeLa Cells Humans Membrane Proteins/chemistry Muscular Dystrophies/pathology Nuclear Envelope/metabolism Nuclear Proteins Protein Binding Thymopoietins/chemistry Transcription, Genetic
Chemicals
Actins Gelsolin Membrane Proteins Nuclear Proteins Thymopoietins emerin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Holaska James M
Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Kowalski Amy K
Wilson Katherine L
Conflict of Interest

The authors have declared that no conflicts of interest exist.

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Article Info
Journal
PLoS biology
Abbr.
PLoS Biol
ISSN
1545-7885
DOI
10.1371/journal.pbio.0020231
Published
2004-09-00
Epub
2004-00-24
Pages
E231
Language
English
Region
United States
NLM ID
101183755
PMCID
PMC509406
Subset
IM
Grants
NCRR NIH HHS · 1S10-RR14702 · United States
NHLBI NIH HHS · T32 HL007227 · United States
NIGMS NIH HHS · F32 GM067397 · United States
NHLBI NIH HHS · T32 HL07227 · United States
NIGMS NIH HHS · R01 GM048646 · United States
NIGMS NIH HHS · R01 GM48646 · United States
External Links
PubMed source DOI: 10.1371/journal.pbio.0020231 PMC Full Text View journal details
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