Home LiteratureArticle Details
PMID: 11030652 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A myosin I isoform in the nucleus.

Science (New York, N.Y.) ·Vol. 290 ·No. 5490 ·2000-10-13 ·Pages 337-41

Pestic-Dragovich L, Stojiljkovic L, Philimonenko AA, Nowak G, Ke Y, Settlage RE, Shabanowitz J, Hunt DF, Hozak P, de Lanerolle P

Abstract

A nuclear isoform of myosin I beta that contains a unique 16-amino acid amino-terminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I beta colocalized with RNA polymerase II in an alpha-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I beta appears to be in a complex with RNA polymerase II and may affect transcription.

MeSH Terms
3T3 Cells Actins/metabolism Amanitins/pharmacology Amino Acid Sequence Animals Base Sequence Cell Nucleus/metabolism Cloning, Molecular Dactinomycin/pharmacology Exons HeLa Cells Humans Mice Microscopy, Confocal Microscopy, Electron Molecular Motor Proteins Molecular Sequence Data Myosins/chemistry,genetics,immunology,metabolism Nucleic Acid Synthesis Inhibitors/pharmacology Precipitin Tests Protein Isoforms/chemistry,genetics,immunology,metabolism RNA/biosynthesis RNA Polymerase II/metabolism Transcription, Genetic
Chemicals
Actins Amanitins Molecular Motor Proteins Nucleic Acid Synthesis Inhibitors Protein Isoforms Dactinomycin RNA RNA Polymerase II Myosins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Pestic-Dragovich L
Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA.
Stojiljkovic L
Philimonenko A A
Nowak G
Ke Y
Settlage R E
Shabanowitz J
Hunt D F
Hozak P
de Lanerolle P
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.290.5490.337
Published
2000-10-13
Pages
337-41
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NIGMS NIH HHS · GM 37537 · United States
NIGMS NIH HHS · GM 56489 · United States
External Links
PubMed source DOI: 10.1126/science.290.5490.337 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2