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PMID: 15319477 Published · ppublish English Journal Article

Arabidopsis formin AtFH6 is a plasma membrane-associated protein upregulated in giant cells induced by parasitic nematodes.

The Plant cell ·Vol. 16 ·No. 9 ·2004-09-00 ·Pages 2529-40

Favery B, Chelysheva LA, Lebris M, Jammes F, Marmagne A, De Almeida-Engler J, Lecomte P, Vaury C, Arkowitz RA, Abad P

Abstract

Plant-parasitic nematodes Meloidogyne spp induce an elaborate permanent feeding site characterized by the redifferentiation of root cells into multinucleate and hypertrophied giant cells. We have isolated by a promoter trap strategy an Arabidopsis thaliana formin gene, AtFH6, which is upregulated during giant cell formation. Formins are actin-nucleating proteins that stimulate de novo polymerization of actin filaments. We show here that three type-I formins were upregulated in giant cells and that the AtFH6 protein was anchored to the plasma membrane and uniformly distributed. Suppression of the budding defect of the Saccharomyces cerevisiae bni1Delta bnr1Delta mutant showed that AtFH6 regulates polarized growth by controlling the assembly of actin cables. Our results suggest that AtFH6 might be involved in the isotropic growth of hypertrophied feeding cells via the reorganization of the actin cytoskeleton. The actin cables would serve as tracks for vesicle trafficking needed for extensive plasma membrane and cell wall biogenesis. Therefore, determining how plant parasitic nematodes modify root cells into giant cells represents an attractive system to identify genes that regulate cell growth and morphogenesis.

MeSH Terms
Actins/metabolism Amino Acid Sequence/genetics Animals Arabidopsis/genetics,metabolism,parasitology Arabidopsis Proteins/genetics,isolation & purification,metabolism Base Sequence/genetics Cell Membrane/genetics,metabolism Cell Wall/genetics,metabolism DNA, Complementary/analysis,genetics Formins Gene Expression Regulation, Plant/genetics Glucuronidase/metabolism Host-Parasite Interactions/genetics Membrane Proteins/genetics,isolation & purification,metabolism Microfilament Proteins/genetics,isolation & purification,metabolism Molecular Sequence Data Mutation/genetics Nematode Infections/genetics,metabolism Phylogeny Saccharomyces cerevisiae/genetics,metabolism Transport Vesicles/genetics,metabolism Tylenchoidea/metabolism Up-Regulation/genetics
Chemicals
Actins Arabidopsis Proteins DNA, Complementary FH6 protein, Arabidopsis Formins Membrane Proteins Microfilament Proteins Glucuronidase
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Favery Bruno
Institut National de la Recherche Agronomique, Unité Mixte de Recherche Interactions Plantes-Microorganismes et Santé Végétale, 06903 Sophia-Antipolis BP167, France.
Chelysheva Liudmila A
Lebris Manuel
Jammes Fabien
Marmagne Anne
De Almeida-Engler Janice
Lecomte Philippe
Vaury Chantal
Arkowitz Robert A
Abad Pierre
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.024372
Published
2004-09-00
Epub
2004-00-19
Pages
2529-40
Language
English
Region
England
NLM ID
9208688
PMCID
PMC520950
Subset
IM
Databases
GENBANK
AAM15566, AF213695, AY337456, L37838, O60610, O94532, P40450, P41832, P48608, P78621, U34258, X62681
RefSeq
XP_109355
External Links
PubMed source DOI: 10.1105/tpc.104.024372 PMC Full Text View journal details
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