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PMID: 11826302 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The Arabidopsis SPIKE1 gene is required for normal cell shape control and tissue development.

The Plant cell ·Vol. 14 ·No. 1 ·2002-01-00 ·Pages 101-18

Qiu JL, Jilk R, Marks MD, Szymanski DB

Abstract

Regulated growth and cell shape control are fundamentally important to the function of plant cells, tissues, and organs. The signal transduction cascades that control localized growth and cell shape, however, are not known. To better understand the relationship between cytoskeletal organization, organelle positioning, and regulated vesicle transport, we conducted a forward genetic screen to identify genes that regulate cytoskeletal organization in plants. Because of the distinct requirements for microtubules and actin filaments during leaf trichome development, a trichome-based morphology screen is an efficient approach to identify genes that affect cytoplasmic organization. The seedling lethal spike1 mutant was identified based on trichome, cotyledon, and leaf-shape defects. The predicted SPIKE1 protein shares amino acid identity with a large family of adapter proteins present in humans, flies, and worms that integrate extracellular signals with cytoskeletal reorganization. Both the trichome phenotype and immunolocalization data suggest that SPIKE1 also is involved in cytoskeletal reorganization. The assembly of laterally clustered foci of microtubules and polarized growth are early events in cotyledon development, and both processes are misregulated in spike1 epidermal cells.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Arabidopsis Proteins/genetics Cell Size/genetics Cell Surface Extensions/genetics Chromosome Mapping Cloning, Molecular Cotyledon/cytology,growth & development Cytoskeleton/genetics,metabolism DNA, Complementary/chemistry,genetics Gene Expression/genetics Microtubules/metabolism Molecular Sequence Data Mutation Phenotype Plant Epidermis/cytology,growth & development Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction/genetics
Chemicals
Arabidopsis Proteins DNA, Complementary SPIKE1 protein, Arabidopsis
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Qiu Jin-Long
Department of Agronomy, Purdue University, 1150 Lilly Hall of Life Sciences, West Lafayette, Indiana 47907-1150, USA.
Jilk Ross
Marks M David
Szymanski Daniel B
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010346
Published
2002-01-00
Pages
101-18
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150554
Subset
IM
Databases
GENBANK
AF465831
External Links
PubMed source DOI: 10.1105/tpc.010346 PMC Full Text View journal details
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