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PMID: 9808621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Myoblast city, the Drosophila homolog of DOCK180/CED-5, is required in a Rac signaling pathway utilized for multiple developmental processes.

Genes & development ·Vol. 12 ·No. 21 ·1998-11-01 ·Pages 3337-42

Nolan KM, Barrett K, Lu Y, Hu KQ, Vincent S, Settleman J

Abstract

The Rac and Cdc42 GTPases share several regulators and effectors, yet perform distinct biological functions. The factors determining such specificity in vivo have not been identified. In a mutational screen in Drosophila to identify Rac-specific signaling components, we isolated 11 alleles of myoblast city (mbc). mbc mutant embryos exhibit defects in dorsal closure, myogenesis, and neural development. DOCK180, the mammalian homolog of Mbc, associates with Rac, but not Cdc42, in a nucleotide-independent manner. These results suggest that Mbc is a specific upstream regulator of Rac activity that mediates several morphogenetic processes in Drosophila embryogenesis.

MeSH Terms
Animals Animals, Genetically Modified COS Cells Caenorhabditis elegans Proteins Cytoskeletal Proteins Drosophila/embryology,genetics Drosophila Proteins GTP Phosphohydrolases/genetics,metabolism,physiology GTP-Binding Proteins/genetics,metabolism,physiology Gene Expression Regulation, Developmental Insect Proteins/genetics,physiology Membrane Proteins/physiology Mutation Proteins/metabolism,physiology Sequence Homology, Amino Acid Signal Transduction/genetics rac GTP-Binding Proteins
Chemicals
CED-5 protein, C elegans Caenorhabditis elegans Proteins Cytoskeletal Proteins DOCK1 protein, human Drosophila Proteins Insect Proteins Membrane Proteins Proteins mbc protein, Drosophila GTP Phosphohydrolases GTP-Binding Proteins rac GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nolan K M
Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown, Massachusetts 02129 USA.
Barrett K
Lu Y
Hu K Q
Vincent S
Settleman J
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.12.21.3337
Published
1998-11-01
Pages
3337-42
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317223
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.12.21.3337 PMC Full Text View journal details
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