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PMID: 9245788 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Drosophila myoblast city encodes a conserved protein that is essential for myoblast fusion, dorsal closure, and cytoskeletal organization.

The Journal of cell biology ·Vol. 138 ·No. 3 ·1997-08-11 ·Pages 589-603

Erickson MR, Galletta BJ, Abmayr SM

Abstract

The Drosophila myoblast city (mbc) locus was previously identified on the basis of a defect in myoblast fusion (Rushton et al., 1995. Development [Camb.]. 121:1979-1988). We describe herein the isolation and characterization of the mbc gene. The mbc transcript and its encoded protein are expressed in a broad range of tissues, including somatic myoblasts, cardial cells, and visceral mesoderm. It is also expressed in the pole cells and in ectodermally derived tissues, including the epidermis. Consistent with this latter expression, mbc mutant embryos exhibit defects in dorsal closure and cytoskeletal organization in the migrating epidermis. Both the mesodermal and ectodermal defects are reminiscent of those induced by altered forms of Drac1 and suggest that mbc may function in the same pathway. MBC bears striking homology to human DOCK180, which interacts with the SH2-SH3 adapter protein Crk and may play a role in signal transduction from focal adhesions. Taken together, these results suggest the possibility that MBC is an intermediate in a signal transduction pathway from the rho/rac family of GTPases to events in the cytoskeleton and that this pathway may be used during myoblast fusion and dorsal closure.

MeSH Terms
Amino Acid Sequence Animals Cell Fusion Cell Movement Cytoskeletal Proteins Cytoskeleton/ultrastructure Drosophila/chemistry,embryology,genetics Drosophila Proteins Ectoderm/metabolism Embryo, Nonmammalian/metabolism Embryonic Development Epidermis/embryology,metabolism Female Gene Expression Regulation, Developmental Genes, Insect Humans Insect Proteins/chemistry,genetics,physiology Male Mesoderm/metabolism Molecular Sequence Data Molecular Weight Muscle Fibers, Skeletal/cytology,metabolism Proteins/chemistry Sequence Alignment Signal Transduction rac GTP-Binding Proteins
Chemicals
Cytoskeletal Proteins DOCK1 protein, human Drosophila Proteins Insect Proteins Proteins mbc protein, Drosophila rac GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Erickson M R
Department of Biochemistry and Molecular Biology and Center for Gene Regulation, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Galletta B J
Abmayr S M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.138.3.589
Published
1997-08-11
Pages
589-603
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2141626
Subset
IM
Grants
NIAMS NIH HHS · R01 AR44274 · United States
Databases
GENBANK
AA110899, AF007805, D86964, Z81032, Z81054
External Links
PubMed source DOI: 10.1083/jcb.138.3.589 PMC Full Text View journal details
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