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PMID: 9843485 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RPE, a plant gene involved in early developmental steps of nematode feeding cells.

The EMBO journal ·Vol. 17 ·No. 23 ·1998-12-01 ·Pages 6799-811

Favery B, Lecomte P, Gil N, Bechtold N, Bouchez D, Dalmasso A, Abad P

Abstract

Sedentary plant-parasitic nematodes are able to induce the redifferentiation of root cells into multinucleate nematode feeding sites (NFSs). We have isolated by promoter trapping an Arabidopsis thaliana gene that is essential for the early steps of NFS formation induced by the root-knot nematode Meloidogyne incognita. Its pattern of expression is similar to that of key regulators of the cell cycle, but it is not observed with the cyst nematode. Later in NFS development, this gene is induced by both root-knot and cyst nematodes. It encodes a protein similar to the D-ribulose-5-phosphate 3-epimerase (RPE) (EC 5.1.3.1), a key enzyme in the reductive Calvin cycle and the oxidative pentose phosphate pathway (OPPP). Quantitative RT-PCR showed the accumulation of RPE transcripts in potato, as in Arabidopsis NFS. Homozygous rpe plants have a germination mutant phenotype that can be rescued in dwarf plants on sucrose-supplemented medium. During root development, this gene is expressed in the meristems and initiation sites of lateral roots. These results suggest that the genetic control of NFSs and the first stages of meristem formation share common steps and confirms the previous cytological observations which indicate that root cells undergo metabolic reprogramming when they turn into NFSs.

MeSH Terms
Amino Acid Sequence Animals Arabidopsis/enzymology,genetics,parasitology Carbohydrate Epimerases/genetics Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Molecular Sequence Data Mutagenesis Nematoda/physiology Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
Carbohydrate Epimerases ribulosephosphate 3-epimerase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Favery B
INRA, Laboratoire de Biologie des Invertébrés, 123 bd F. Meilland, 06600 Antibes, France.
Lecomte P
Gil N
Bechtold N
Bouchez D
Dalmasso A
Abad P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.23.6799
Published
1998-12-01
Pages
6799-811
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1171027
Subset
IM
Databases
GENBANK
AF015274, AF047444
External Links
PubMed source DOI: 10.1093/emboj/17.23.6799 PMC Full Text View journal details
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