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PMID: 15138768 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TopBP1 localises to centrosomes in mitosis and to chromosome cores in meiosis.

Chromosoma ·Vol. 112 ·No. 7 ·2004-05-00 ·Pages 323-30

Reini K, Uitto L, Perera D, Moens PB, Freire R, Syväoja JE

Abstract

Topoisomerase IIbeta binding protein 1 (TopBP1), previously shown to localise to sites of DNA damage and to stalled replication forks, has been implicated in DNA replication and in DNA damage response. In this work we showed that TopBP1 was localised in structures other than stalled replication forks. In late mitosis TopBP1 localises to centrosomes in a manner similar to other DNA damage response proteins such as BRCA1 and p53. Spindle checkpoint activation does not affect this centrosomal localisation. Moreover, in the testis, we detected high levels of TopBP1 associated with meiotic prophase chromosome cores and the X-Y pair. Together, these data suggest a direct role of TopBP1 during both mitosis and meiotic prophase I.

MeSH Terms
Anaphase BRCA1 Protein/analysis,genetics Carrier Proteins/analysis,genetics,physiology Cell Line, Tumor Centrosome/chemistry Chromosomes/chemistry,ultrastructure DNA-Binding Proteins Enzyme Activation Female HeLa Cells Humans Male Meiotic Prophase I Metaphase Mitosis Nocodazole/pharmacology Nuclear Proteins Pyrimidines/pharmacology Spermatogenesis Testis/chemistry,cytology Thiones/pharmacology Time Factors
Chemicals
BRCA1 Protein Carrier Proteins DNA-Binding Proteins Nuclear Proteins Pyrimidines TOPBP1 protein, human Thiones monastrol Nocodazole
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Reini Kaarina
Biocenter Oulu and Department of Biochemistry, P.O. Box 3000, 90014, University of Oulu, Finland.
Uitto Lahja
Perera David
Moens Peter B
Freire Raimundo
Syväoja Juhani E
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/s00412-004-0277-5
Published
2004-05-00
Epub
2004-00-06
Pages
323-30
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/s00412-004-0277-5 View journal details
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