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PMID: 9037052 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Evidence for a role for DNA polymerase beta in mammalian meiosis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 4 ·1997-02-18 ·Pages 1327-31

Plug AW, Clairmont CA, Sapi E, Ashley T, Sweasy JB

Abstract

DNA polymerase beta (pol beta) is an enzyme possessing both polymerase and deoxyribose phophatase activities. Although pol beta is not believed to participate in the replication of genomic DNA, several studies have indicated a role for pol beta in DNA repair. The high level of expression of pol beta in mouse and rat testes raises the possibility that pol beta participates in mammalian meiosis. Using antibody localization, we detect foci that stain with pol beta antisera at discrete sites along homologous chromosomes as they synapse and progress through prophase of meiosis I. These data suggest that pol beta participates in meiotic events associated with synapsis and recombination.

MeSH Terms
Animals Cell Differentiation Chromosomes/enzymology DNA Polymerase I/immunology,isolation & purification Immunohistochemistry Male Mice Recombination, Genetic Spermatocytes/enzymology Synaptonemal Complex Testis/enzymology
Chemicals
DNA Polymerase I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Plug A W
Department of Genetics, Yale University School of Medicine, New Haven, CT 06520, USA.
Clairmont C A
Sapi E
Ashley T
Sweasy J B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.4.1327
Published
1997-02-18
Pages
1327-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC19790
Subset
IM
Grants
NCI NIH HHS · F32 CA068764 · United States
NCI NIH HHS · CA-64134 · United States
NCI NIH HHS · CA-68764 · United States
NIGMS NIH HHS · GM-49799 · United States
External Links
PubMed source DOI: 10.1073/pnas.94.4.1327 PMC Full Text View journal details
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