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PMID: 14996934 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nuclear location of a chromatin insulator in Drosophila melanogaster.

Journal of cell science ·Vol. 117 ·No. Pt 7 ·2004-03-01 ·Pages 1025-32

Xu Q, Li M, Adams J, Cai HN

Abstract

Chromatin-related functions are associated with spatial organization in the nucleus. We have investigated the relationship between the enhancer-blocking activity and subnuclear localization of the Drosophila melanogaster suHw insulator. Using fluorescent in situ hybridization, we observed that genomic loci containing the gypsy retrotransposon were distributed closer to the nuclear periphery than regions without the gypsy retrotransposon. However, transgenes containing a functional 340 bp suHw insulator did not exhibit such biased distribution towards the nuclear periphery, which suggests that the suHw insulator sequence is not responsible for the peripheral localization of the gypsy retrotransposon. Antibody stains showed that the two proteins essential for the suHw insulator activity, SUHW and MOD(MDG4), are not restricted to the nuclear periphery. The enhancer-blocking activity of suHw remained intact under the heat shock conditions, which was shown to disrupt the association of gypsy, SUHW and MOD(MDG4) with the nuclear periphery. Our results indicate that the suHw insulator can function in the nuclear interior, possibly through local interactions with chromatin components or other nuclear structures.

MeSH Terms
Animals Animals, Genetically Modified Base Sequence Cell Nucleus/metabolism Chromatin/metabolism DNA/genetics DNA-Binding Proteins/genetics,metabolism Drosophila Proteins/genetics,metabolism Drosophila melanogaster/genetics,metabolism In Situ Hybridization, Fluorescence Insulator Elements Lac Operon Nuclear Proteins/genetics,metabolism Recombinant Proteins/genetics,metabolism Repressor Proteins Retroelements Transcription Factors/genetics,metabolism
Chemicals
Chromatin DNA-Binding Proteins Drosophila Proteins Nuclear Proteins Recombinant Proteins Repressor Proteins Retroelements Transcription Factors mdg4 protein (gypsy) su(Hw) protein, Drosophila DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Xu Qinghao
Department of Cellular Biology, The University of Georgia, Athens, GA 30602, USA.
Li Mo
Adams Jessica
Cai Haini N
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Article Info
Journal
Journal of cell science
Abbr.
J Cell Sci
ISSN
0021-9533
DOI
10.1242/jcs.00964
Published
2004-03-01
Pages
1025-32
Language
English
Region
England
NLM ID
0052457
PMCID
PMC4334384
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058458 · United States
External Links
PubMed source DOI: 10.1242/jcs.00964 PMC Full Text View journal details
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