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PMID: 12473685 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Gene density and transcription influence the localization of chromatin outside of chromosome territories detectable by FISH.

The Journal of cell biology ·Vol. 159 ·No. 5 ·2002-12-09 ·Pages 753-63

Mahy NL, Perry PE, Bickmore WA

Abstract

Genes can be transcribed from within chromosome territories; however, the major histocompatibilty complex locus has been reported extending away from chromosome territories, and the incidence of this correlates with transcription from the region. A similar result has been seen for the epidermal differentiation complex region of chromosome 1. These data suggested that chromatin decondensation away from the surface of chromosome territories may result from, and/or may facilitate, transcription of densely packed genes subject to coordinate regulation.To investigate whether localization outside of the visible confines of chromosome territories can also occur for regions that are not coordinately regulated, we have examined the spatial organization of human 11p15.5 and the syntenic region on mouse chromosome 7. This region is gene rich but its genes are not coordinately expressed, rather overall high levels of transcription occur in several cell types. We found that chromatin from 11p15.5 frequently extends away from the chromosome 11 territory. Localization outside of territories was also detected for other regions of high gene density and high levels of transcription. This is shown to be partly dependent on ongoing transcription. We suggest that local gene density and transcription, rather than the activity of individual genes, influences the organization of chromosomes in the nucleus.

MeSH Terms
Animals Cell Nucleus/drug effects,genetics Cells, Cultured Chromatin/chemistry,genetics,metabolism Chromosome Painting Chromosomes, Artificial, P1 Bacteriophage/genetics Chromosomes, Human, Pair 11/genetics,metabolism Chromosomes, Mammalian/genetics,metabolism Cosmids/genetics DNA/genetics,metabolism Dactinomycin/pharmacology Dichlororibofuranosylbenzimidazole/pharmacology Fibroblasts/cytology,drug effects,metabolism Genes Genetic Markers Genome, Human Humans In Situ Hybridization, Fluorescence/methods Lymphocyte Activation Lymphocytes/cytology,drug effects,metabolism Mice Nucleic Acid Synthesis Inhibitors/pharmacology Physical Chromosome Mapping Synteny Telomere/chemistry Transcription, Genetic
Chemicals
Chromatin Genetic Markers Nucleic Acid Synthesis Inhibitors Dactinomycin Dichlororibofuranosylbenzimidazole DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mahy Nicola L
MRC Human Genetics Unit, Edinburgh EH4 2XU, UK.
Perry Paul E
Bickmore Wendy A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200207115
Published
2002-12-09
Epub
2002-00-09
Pages
753-63
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173389
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200207115 PMC Full Text View journal details
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