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PMID: 1494351 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intramolecular homologous recombination in Bacillus subtilis 168.

Molecular & general genetics : MGG ·Vol. 236 ·No. 1 ·1992-12-00 ·Pages 60-4

Alonso JC, Lüder G, Trautner TA

Abstract

Plasmid resolution from a phage::plasmid chimera was used to measure directly intramolecular recombination in Bacillus subtilis. The system is based on a sigma-replicating plasmid (pC194) cloned into a dispensable region of the lytic bacteriophage SPP1. The plasmid, which confers chloramphenicol resistance, is resolved when SPP1::pC194 phages infect B. subtilis cells, provided the chimera carries a functional, intact copy of the plasmid repH gene. Intramolecular homologous recombination was independent of the RecA and RecL-RecR functions, but dependent on RecF, RecB, RecG, RecP, RecH and AddAB functions. These results are consistent with the hypothesis that B. subtilis has multiple pathways for genetic recombination and allow us to tentatively place the recB and recG genes into a new epistatic group epsilon.

Related Genes
rec repH
MeSH Terms
Bacillus subtilis/genetics Bacteriophages/genetics Base Sequence Chimera DNA, Recombinant Genes, Bacterial Genes, Viral Molecular Sequence Data Plasmids Recombination, Genetic
Chemicals
DNA, Recombinant
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Alonso J C
Max-Planck-Institut für molekulare Genetik, Berlin, FRG.
Lüder G
Trautner T A
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00279643
Published
1992-12-00
Pages
60-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00279643
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