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PMID: 14715908 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Coregulator-dependent facilitation of chromatin occupancy by GATA-1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 4 ·2004-01-27 ·Pages 980-5

Pal S, Cantor AB, Johnson KD, Moran TB, Boyer ME, Orkin SH, Bresnick EH

Abstract

Coregulator recruitment by DNA-bound factors results in chromatin modification and protein-protein interactions, which regulate transcription. However, the mechanism by which the Friend of GATA (FOG) coregulator mediates GATA factor-dependent transcription is unknown. We showed previously that GATA-1 replaces GATA-2 at an upstream region of the GATA-2 locus, and that this GATA switch represses GATA-2. Genetic complementation analysis in FOG-1-null hematopoietic precursors revealed that FOG-1 is not required for establishment or maintenance of the active GATA-2 domain, but is critical for the GATA switch. Analysis of GATA factor binding to additional loci also revealed FOG-1-dependent GATA switches. Thus, FOG-1 facilitates chromatin occupancy by GATA-1 at sites bound by GATA-2. We propose that FOG-1 is a prototype of a new class of coregulators termed chromatin occupancy facilitators, which confer coregulation in certain contexts via enhancing trans-acting factor binding to chromatin in vivo.

MeSH Terms
Carrier Proteins/physiology Cell Line, Transformed Chromatin/metabolism DNA-Binding Proteins/genetics,physiology Erythroid-Specific DNA-Binding Factors GATA2 Transcription Factor Nuclear Proteins/physiology Reverse Transcriptase Polymerase Chain Reaction Transcription Factors/genetics,physiology Transcription, Genetic
Chemicals
Carrier Proteins Chromatin DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors GATA2 Transcription Factor Nuclear Proteins Transcription Factors ZFPM1 protein, human
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pal Saumen
Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, 383 Medical Sciences Center, Madison, WI 53706, USA.
Cantor Alan B
Johnson Kirby D
Moran Tyler B
Boyer Meghan E
Orkin Stuart H
Bresnick Emery H
References (45)
45 references, click to expand
  1. Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes.
    EMBO J. 2000 Jun 1;19(11):2629-40 PMID: 10835360
  2. Use of altered specificity mutants to probe a specific protein-protein interaction in differentiation: the GATA-1:FOG complex.
    Mol Cell. 1999 Feb;3(2):219-28 PMID: 10078204
  3. Role of zinc finger domains of the transcription factor neuron-restrictive silencer factor/repressor element-1 silencing transcription factor in DNA binding and nuclear localization.
    J Biol Chem. 2001 Apr 20;276(16):13121-6 PMID: 11145971
  4. Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4.
    Mol Cell. 2002 Feb;9(2):279-89 PMID: 11864602
  5. Interaction between FOG-1 and the corepressor C-terminal binding protein is dispensable for normal erythropoiesis in vivo.
    Mol Cell Biol. 2002 May;22(9):3121-8 PMID: 11940669
  6. Distinct domains of the GATA-1 cofactor FOG-1 differentially influence erythroid versus megakaryocytic maturation.
    Mol Cell Biol. 2002 Jun;22(12):4268-79 PMID: 12024038
  7. Essential and instructive roles of GATA factors in eosinophil development.
    J Exp Med. 2002 Jun 3;195(11):1379-86 PMID: 12045236
  8. Targeted deletion of a high-affinity GATA-binding site in the GATA-1 promoter leads to selective loss of the eosinophil lineage in vivo.
    J Exp Med. 2002 Jun 3;195(11):1387-95 PMID: 12045237
  9. GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis.
    Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9237-42 PMID: 12077323
  10. Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain.
    Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11760-5 PMID: 12193659
  11. Hematopoietic-specific activators establish an overlapping pattern of histone acetylation and methylation within a mammalian chromatin domain.
    Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14309-14 PMID: 12379744
  12. Deletion of the mouse alpha-globin regulatory element (HS -26) has an unexpectedly mild phenotype.
    Blood. 2002 Nov 15;100(10):3450-6 PMID: 12393394
  13. GATA-1-dependent transcriptional repression of GATA-2 via disruption of positive autoregulation and domain-wide chromatin remodeling.
    Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8811-6 PMID: 12857954
  14. Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
    EMBO J. 1985 Dec 16;4(13A):3473-82 PMID: 4092686
  15. The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing.
    Cell. 1987 Jan 16;48(1):91-100 PMID: 3791417
  16. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  17. The erythroid-specific transcription factor Eryf1: a new finger protein.
    Cell. 1989 Sep 8;58(5):877-85 PMID: 2776214
  18. Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome.
    Science. 1990 Feb 16;247(4944):824-30 PMID: 2406902
  19. Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1.
    Nature. 1991 Jan 17;349(6306):257-60 PMID: 1987478
  20. Transcriptional activation of human zeta 2 globin promoter by the alpha globin regulatory element (HS-40): functional role of specific nuclear factor-DNA complexes.
    Mol Cell Biol. 1993 Apr;13(4):2298-308 PMID: 8455611
  21. Rescue of erythroid development in gene targeted GATA-1- mouse embryonic stem cells.
    Nat Genet. 1992 May;1(2):92-8 PMID: 1302015
  22. DNA-binding specificity of GATA family transcription factors.
    Mol Cell Biol. 1993 Jul;13(7):3999-4010 PMID: 8321207
  23. DNA-binding specificities of the GATA transcription factor family.
    Mol Cell Biol. 1993 Jul;13(7):4011-22 PMID: 8321208
  24. Different sequence requirements for expression in erythroid and megakaryocytic cells within a regulatory element upstream of the GATA-1 gene.
    Development. 1999 Jun;126(12):2799-811 PMID: 10331989
  25. GATA-1 and erythropoietin cooperate to promote erythroid cell survival by regulating bcl-xL expression.
    Blood. 1999 Jul 1;94(1):87-96 PMID: 10381501
  26. Dynamics of GATA transcription factor expression during erythroid differentiation.
    Blood. 1993 Aug 15;82(4):1071-9 PMID: 8353273
  27. An early haematopoietic defect in mice lacking the transcription factor GATA-2.
    Nature. 1994 Sep 15;371(6494):221-6 PMID: 8078582
  28. Localization and characterization of the mouse alpha-globin locus control region.
    Genomics. 1994 May 15;21(2):431-3 PMID: 8088839
  29. Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells.
    Genes Dev. 1994 May 15;8(10):1184-97 PMID: 7926723
  30. GATA transcription factors: key regulators of hematopoiesis.
    Exp Hematol. 1995 Feb;23(2):99-107 PMID: 7828675
  31. Pluripotent hematopoietic stem cells contain high levels of mRNA for c-kit, GATA-2, p45 NF-E2, and c-myb and low levels or no mRNA for c-fms and the receptors for granulocyte colony-stimulating factor and interleukins 5 and 7.
    Proc Natl Acad Sci U S A. 1995 May 9;92(10):4601-5 PMID: 7538677
  32. Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation.
    Cell. 1996 Mar 22;84(6):843-51 PMID: 8601308
  33. X-MyT1, a Xenopus C2HC-type zinc finger protein with a regulatory function in neuronal differentiation.
    Cell. 1996 Dec 27;87(7):1191-202 PMID: 8980226
  34. Erythroid-cell-specific properties of transcription factor GATA-1 revealed by phenotypic rescue of a gene-targeted cell line.
    Mol Cell Biol. 1997 Mar;17(3):1642-51 PMID: 9032291
  35. Comparative analysis of genetically modified dendritic cells and tumor cells as therapeutic cancer vaccines.
    J Exp Med. 2000 May 15;191(10):1699-708 PMID: 10811863
  36. Histone deacetylases associated with the mSin3 corepressor mediate mad transcriptional repression.
    Cell. 1997 May 2;89(3):349-56 PMID: 9150134
  37. Transcription factor GATA-2 is required for proliferation/survival of early hematopoietic cells and mast cell formation, but not for erythroid and myeloid terminal differentiation.
    Blood. 1997 May 15;89(10):3636-43 PMID: 9160668
  38. FOG, a multitype zinc finger protein, acts as a cofactor for transcription factor GATA-1 in erythroid and megakaryocytic differentiation.
    Cell. 1997 Jul 11;90(1):109-19 PMID: 9230307
  39. Sequence-specific DNA binding and transcriptional regulation by the promyelocytic leukemia zinc finger protein.
    J Biol Chem. 1997 Sep 5;272(36):22447-55 PMID: 9278395
  40. Alternative promoters regulate transcription of the mouse GATA-2 gene.
    J Biol Chem. 1998 Feb 6;273(6):3625-34 PMID: 9452491
  41. CREB-binding protein cooperates with transcription factor GATA-1 and is required for erythroid differentiation.
    Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2061-6 PMID: 9482838
  42. Failure of megakaryopoiesis and arrested erythropoiesis in mice lacking the GATA-1 transcriptional cofactor FOG.
    Genes Dev. 1998 Apr 15;12(8):1176-88 PMID: 9553047
  43. Identification and characterization of a conserved erythroid-specific enhancer located in intron 8 of the human 5-aminolevulinate synthase 2 gene.
    J Biol Chem. 1998 Jul 3;273(27):16798-809 PMID: 9642238
  44. Perturbation of nucleosome structure by the erythroid transcription factor GATA-1.
    J Mol Biol. 1998 Jun 12;279(3):529-44 PMID: 9641976
  45. The zinc finger-containing transcription factors GATA-4, -5, and -6. Ubiquitously expressed regulators of tissue-specific gene expression.
    J Biol Chem. 2000 Dec 15;275(50):38949-52 PMID: 11042222
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0307612100
Published
2004-01-27
Epub
2004-00-08
Pages
980-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC327128
Subset
IM
Grants
NHLBI NIH HHS · T32 HL007936 · United States
NCI NIH HHS · K08 CA82175-05 · United States
NIDDK NIH HHS · DK55700 · United States
NIDDK NIH HHS · R01 DK050107 · United States
NIDDK NIH HHS · R01 DK055700 · United States
NHLBI NIH HHS · T32 HL07899 · United States
NIDDK NIH HHS · DK50107 · United States
NHLBI NIH HHS · T32 HL007899 · United States
NIDDK NIH HHS · R37 DK050107 · United States
NHLBI NIH HHS · T32 HL07936 · United States
External Links
PubMed source DOI: 10.1073/pnas.0307612100 PMC Full Text View journal details
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