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PMID: 11864602 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4.

Molecular cell ·Vol. 9 ·No. 2 ·2002-02-00 ·Pages 279-89

Cirillo LA, Lin FR, Cuesta I, Friedman D, Jarnik M, Zaret KS

Abstract

The transcription factors HNF3 (FoxA) and GATA-4 are the earliest known to bind the albumin gene enhancer in liver precursor cells in embryos. To understand how they access sites in silent chromatin, we assembled nucleosome arrays containing albumin enhancer sequences and compacted them with linker histone. HNF3 and GATA-4, but not NF-1, C/EBP, and GAL4-AH, bound their sites in compacted chromatin and opened the local nucleosomal domain in the absence of ATP-dependent enzymes. The ability of HNF3 to open chromatin is mediated by a high affinity DNA binding site and by the C-terminal domain of the protein, which binds histones H3 and H4. Thus, factors that potentiate transcription in development are inherently capable of initiating chromatin opening events.

MeSH Terms

Amino Acid Motifs Animals Binding Sites CCAAT-Enhancer-Binding Protein-beta/metabolism Chromatin/genetics,metabolism,ultrastructure DNA-Binding Proteins/chemistry,physiology Endoderm/metabolism Enhancer Elements, Genetic/physiology Fetal Proteins/biosynthesis,genetics,physiology GATA4 Transcription Factor Gene Expression Regulation, Developmental/physiology Gene Silencing Hepatocyte Nuclear Factor 3-alpha Histones/physiology Liver/embryology,metabolism Macromolecular Substances Mice Models, Genetic Neurofibromin 1/metabolism Nuclear Proteins/chemistry,physiology Nucleosomes/physiology Promoter Regions, Genetic/physiology Recombinant Fusion Proteins/physiology Serum Albumin/biosynthesis,genetics Structure-Activity Relationship Transcription Factors/physiology Transcription, Genetic/physiology

Chemicals

CCAAT-Enhancer-Binding Protein-beta Chromatin DNA-Binding Proteins Fetal Proteins Foxa1 protein, mouse GATA4 Transcription Factor Hepatocyte Nuclear Factor 3-alpha Histones Macromolecular Substances Neurofibromin 1 Nuclear Proteins Nucleosomes Recombinant Fusion Proteins Serum Albumin Transcription Factors

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Cirillo Lisa Ann

Cell and Developmental Biology Program, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA.

Lin Frank Robert

Cuesta Isabel

Friedman Dara

Jarnik Michal

Zaret Kenneth S

Article Info

Journal

Molecular cell

Abbr.

Mol Cell

ISSN

1097-2765

DOI

10.1016/s1097-2765(02)00459-8

Published

2002-02-00

Pages

279-89

Language

English

Region

United States

NLM ID

9802571

Subset

Grants

NCI NIH HHS · CA06297 · United States

NIDDK NIH HHS · DK09529-01 · United States

NIGMS NIH HHS · GM47903 · United States

Corrections

CommentIn

PMID: 11864594

External Links

PubMed source DOI: 10.1016/s1097-2765(02)00459-8 View journal details

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Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4.

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