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PMID: 12193659 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 18 ·2002-09-03 ·Pages 11760-5

Johnson KD, Grass JA, Boyer ME, Kiekhaefer CM, Blobel GA, Weiss MJ, Bresnick EH

Abstract

The hematopoietic transcription factor GATA-1 regulates erythropoiesis and beta-globin expression. Although consensus GATA-1 binding sites exist throughout the murine beta-globin locus, we found that GATA-1 discriminates among these sites in vivo. Conditional expression of GATA-1 in GATA-1-null cells recapitulated the occupancy pattern. GATA-1 induced RNA polymerase II (pol II) recruitment to subregions of the locus control region and to the beta-globin promoters. The hematopoietic factor NF-E2 cooperated with GATA-1 to recruit pol II to the promoters. We propose that only when GATA-1 attracts pol II to the locus control region can pol II access the promoter in a NF-E2-dependent manner.

MeSH Terms
Animals Bone Marrow/metabolism Chromatin/metabolism DNA-Binding Proteins/metabolism Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Globins/genetics Mice NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Promoter Regions, Genetic RNA Polymerase II/metabolism Transcription Factors/metabolism Tumor Cells, Cultured
Chemicals
Chromatin DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor Gata1 protein, mouse NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit Nfe2 protein, mouse Transcription Factors Globins RNA Polymerase II
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Johnson Kirby D
Department of Pharmacology, Molecular and Cellular Pharmacology Program, University of Wisconsin Medical School, 383 Medical Science Center, 1300 University Avenue, Madison, WI 53706, USA.
Grass Jeffrey A
Boyer Meghan E
Kiekhaefer Carol M
Blobel Gerd A
Weiss Mitchell J
Bresnick Emery H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.192285999
Published
2002-09-03
Epub
2002-00-22
Pages
11760-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC129342
Subset
IM
Grants
NIDDK NIH HHS · DK55700 · United States
NIDDK NIH HHS · R01 DK050107 · United States
NIDDK NIH HHS · R01 DK055700 · United States
NIDDK NIH HHS · DK50107 · United States
NIDDK NIH HHS · R37 DK050107 · United States
External Links
PubMed source DOI: 10.1073/pnas.192285999 PMC Full Text View journal details
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