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PMID: 2209547 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Site-specific recombination of the tal-1 gene is a common occurrence in human T cell leukemia.

The EMBO journal ·Vol. 9 ·No. 10 ·1990-10-00 ·Pages 3343-51

Brown L, Cheng JT, Chen Q, Siciliano MJ, Crist W, Buchanan G, Baer R

Abstract

The tal-1 gene is altered as a consequence of the t(1;14) (p32;q11) chromosome translocation observed in 3% of patients with T cell acute lymphoblastic leukemia (T-ALL). tal-1 encodes a helix-loop-helix (HLH) domain, a DNA binding and dimerization motif found in a number of proteins involved in cell growth and differentiation. We now report that an additional 25% of T-ALL patients bear tal-1 gene rearrangements that are not detected by karyotype analysis. These rearrangements result from a precise 90 kb deletion (designated tald) that arises independently in different patients by site-specific DNA recombination. Since the deletion junctions resemble the coding joints of assembled immunoglobulin genes, tald rearrangements are likely to be mediated by aberrant activity of the immunoglobulin recombinase. Moreover, t(1;14)(p32;q11) translocations and tald rearrangements disrupt the coding potential of tal-1 in an equivalent manner, and thereby generate a common genetic lesion shared by a significant proportion of T-ALL patients.

Related Genes
tal-1
MeSH Terms
Base Sequence Chromosome Deletion Chromosomes, Human, Pair 1 Chromosomes, Human, Pair 14 Cloning, Molecular Gene Rearrangement Humans Leukemia-Lymphoma, Adult T-Cell/genetics Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction Recombination, Genetic Restriction Mapping Translocation, Genetic
Chemicals
Oligonucleotide Probes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Brown L
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas.
Cheng J T
Chen Q
Siciliano M J
Crist W
Buchanan G
Baer R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-10-00
Pages
3343-51
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552072
Subset
IM
Grants
NCI NIH HHS · CA-21765 · United States
NCI NIH HHS · CA46593 · United States
NCI NIH HHS · CA47975 · United States
External Links
PubMed source PMC Full Text View journal details
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