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PMID: 14645274 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

FarR regulates the farAB-encoded efflux pump of Neisseria gonorrhoeae via an MtrR regulatory mechanism.

Journal of bacteriology ·Vol. 185 ·No. 24 ·2003-12-00 ·Pages 7145-52

Lee EH, Rouquette-Loughlin C, Folster JP, Shafer WM

Abstract

The farAB operon of Neisseria gonorrhoeae encodes an efflux pump which mediates gonococcal resistance to antibacterial fatty acids. It was previously observed that expression of the farAB operon was positively regulated by MtrR, which is a repressor of the mtrCDE-encoded efflux pump system (E.-H. Lee and W. M. Shafer, Mol. Microbiol. 33:839-845, 1999). This regulation was believed to be indirect since MtrR did not bind to the farAB promoter. In this study, computer analysis of the gonococcal genome sequence database, lacZ reporter fusions, and gel mobility shift assays were used to elucidate the regulatory mechanism by which expression of the farAB operon is modulated by MtrR in gonococci. We identified a regulatory protein belonging to the MarR family of transcriptional repressors and found that it negatively controls expression of farAB by directly binding to the farAB promoter. We designated this regulator FarR to signify its role in regulating the farAB operon. We found that MtrR binds to the farR promoter, thereby repressing farR expression. Hence, MtrR regulates farAB in a positive fashion by modulating farR expression. This MtrR regulatory cascade seems to play an important role in adjusting levels of the FarAB and MtrCDE efflux pumps to prevent their excess expression in gonococci.

MeSH Terms
Amino Acid Transport Systems, Neutral/metabolism Bacterial Proteins DNA-Binding Proteins/genetics,metabolism Escherichia coli Proteins/genetics,metabolism Ferredoxin-NADP Reductase Gene Expression Regulation, Bacterial Lac Operon Neisseria gonorrhoeae/genetics,metabolism Promoter Regions, Genetic/physiology Repressor Proteins/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
Amino Acid Transport Systems, Neutral Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Repressor Proteins Transcription Factors mngR protein, E coli mtrR protein, Neisseria gonorrhoeae methionine synthase reductase Ferredoxin-NADP Reductase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee E-H
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Rouquette-Loughlin C
Folster J P
Shafer W M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.24.7145-7152.2003
Published
2003-12-00
Pages
7145-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC296254
Subset
IM
Grants
NIAID NIH HHS · R01 AI021150 · United States
NCRR NIH HHS · RR13948 · United States
NIAID NIH HHS · 5T32 AI-07470 · United States
NIAID NIH HHS · AI-38399 · United States
NIAID NIH HHS · R37 AI021150 · United States
NCRR NIH HHS · RR12878 · United States
NIAID NIH HHS · AI-37945 · United States
NIAID NIH HHS · AI-21150-17 · United States
NCRR NIH HHS · RR02878 · United States
NIAID NIH HHS · P01 AI037945 · United States
NIAID NIH HHS · T32 AI007470 · United States
External Links
PubMed source DOI: 10.1128/JB.185.24.7145-7152.2003 PMC Full Text View journal details
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