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PMID: 14522950 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ELAV inhibits 3'-end processing to promote neural splicing of ewg pre-mRNA.

Genes & development ·Vol. 17 ·No. 20 ·2003-10-15 ·Pages 2526-38

Soller M, White K

Abstract

The embryonic lethal abnormal visual system (ELAV) is a gene-specific regulator of alternative pre-mRNA processing in neurons of Drosophila. Here we define a functional in vivo binding site for ELAV in neurons through the development of a reporter gene system in transgenic animals in combination with in vitro binding assays. ELAV binds to erect wing (ewg) RNA 3' of a polyadenylation site in the terminal intron 6. At this polyadenylation site, ELAV inhibits 3'-end processing in vitro in a dose-dependent and sequence-specific manner, and ELAV binding is necessary in vivo to promote splicing of ewg intron 6. Further, the AAUAAA poly(A) complex recognition sequence, together with ELAV, is required to regulate neural 3' splice site choice in vivo. In addition, the use of segmentally labeled RNA substrates in UV cross-linking assays suggest that ELAV does not inhibit or redirect binding of cleavage factor dCstF64 at the regulated polyadenylation site on ewg RNA. These data indicate that binding of 3'-end processing factors, together with ELAV, can regulate alternative splicing.

MeSH Terms
Adenosine/metabolism Drosophila Proteins ELAV Proteins Genes, Reporter Neurons/metabolism Neuropeptides/genetics,metabolism Nuclear Proteins/genetics,metabolism Polymers/metabolism RNA 3' Polyadenylation Signals RNA Precursors/metabolism RNA Processing, Post-Transcriptional Ribonucleoproteins/metabolism Transcription Factors
Chemicals
Drosophila Proteins ELAV Proteins EWG protein, Drosophila Neuropeptides Nuclear Proteins Polymers RNA Precursors Ribonucleoproteins Transcription Factors polyadenosine Adenosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Soller Matthias
Department of Biology and Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454, USA. msoller@brandeis.edu
White Kalpana
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1106703
Email
msoller@brandeis.edu
Published
2003-10-15
Epub
2003-00-01
Pages
2526-38
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC218147
Subset
IM
Grants
NINDS NIH HHS · P01 NS044232 · United States
NINDS NIH HHS · NS37169 · United States
NINDS NIH HHS · NS44232 · United States
External Links
PubMed source DOI: 10.1101/gad.1106703 PMC Full Text View journal details
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