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PMID: 1447149 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Escherichia coli B lacks one of the two initiator tRNA species present in E. coli K-12.

Journal of bacteriology ·Vol. 174 ·No. 23 ·1992-12-00 ·Pages 7827-30

Mandal N, RajBhandary UL

Abstract

We show that the metY locus which specifies tRNA(2fMet) in Escherichia coli K-12 specifies tRNA(1fMet) in E. coli B. This conclusion is based on results of Southern blot analysis of E. coli B and K-12 DNAs and on polymerase chain reaction amplification, cloning, and sequencing of an approximately 200-bp region of DNA corresponding to the metY loci of E. coli B and E. coli K-12. We also show that the metY locus of E. coli B is transcriptionally active. E. coli strains transformed with the multicopy plasmid vector pUC19 carrying the metY locus of E. coli B overproduce tRNA(1fMet) in E. coli B and E. coli K-12 in contrast to strains transformed with pUC19 carrying the corresponding locus from E. coli K-12, which overproduce tRNA(2fMet).

Related Genes
metY
MeSH Terms
Base Sequence Blotting, Southern Cloning, Molecular Escherichia coli/chemistry,genetics Molecular Sequence Data N-Formylmethionine/metabolism Nucleic Acid Conformation Polymerase Chain Reaction RNA, Transfer, Met/analysis,genetics Transcription, Genetic
Chemicals
RNA, Transfer, Met N-Formylmethionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mandal N
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
RajBhandary U L
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.23.7827-7830.1992
Published
1992-12-00
Pages
7827-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207499
Subset
IM
Grants
NIGMS NIH HHS · GM17151 · United States
External Links
PubMed source DOI: 10.1128/jb.174.23.7827-7830.1992 PMC Full Text View journal details
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